Soil particle size fractions affect arsenic (As) release and speciation: Insights into dissolved organic matter and functional genes.

Soil particle size fractions (PSFs) are important for arsenic (As) partitioning, migration, and speciation transformation. However, information is lacking about the environmental fate of As and its distribution on different PSFs. In the present study, two types of soils from mining areas were divided into four PSFs, including coarse sand (2-0.25 mm), fine sand (0.25-0.05 mm), silt (0.05-0.002 mm), and clay (< 0.002 mm) fractions. The results showed that As was enriched in the coarse sand, which was primarily affected by the content of organic carbon (OC), followed by iron (Fe), aluminum (Al), and manganese (Mn) (hydr)oxides. The elevated total As (TAs), As(III), organic As, Fe(II), and dissolved organic carbon (DOC) concentrations were mainly originated from the clay fraction. The intensified humification degree of DOM and promoted bacterial metabolism related to As/iron bioreduction were also exhibited in the clay fractions. The dynamics of As fractions in soils indicated the potential formation of secondary minerals and re-adsorption of As in the PSFs. The highest abundances of arrA, arsC, arsM, and Geo genes were found in the clay fraction, implying that the clay fraction potentially released more As, including As(III) and organic As. Results from the correlation analysis showed that elevated DOC concentrations promoted the catabolic responses of iron-reducing microorganisms and triggered microbial As detoxification. Overall, this study provides valuable information and guidance for the remediation of As-contaminated soils.