National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Zhejiang Hangzhou Tiao Wang Biological Technology Co., Ltd., Hangzhou, 310015, China.
Fish Shellfish Immunol. 2022 Dec;131:891-897. doi: 10.1016/j.fsi.2022.11.002. Epub 2022 Nov 9.
This study aimed to investigate the effects of dietary Bovine lactoferricin (LFcinB) on the growth performance and non-specific immunity in Macrobrachium rosenbergii. Five experimental diets were 1.0‰ Bovine lactoferricin (LCB1); 1.5‰ Bovine lactoferricin (LCB1.5); 2.0‰ Bovine lactoferricin (LCB2); 2.5‰ Bovine lactoferricin (LCB2.5); the control group, basal diet without Bovine lactoferricin. A total of 600 prawns were randomly assigned to 5 groups in triplicate in 15 tanks for an 8-week feeding trial. The results showed the final weight, weight gain rate, specific growth rate and survival rate of prawns in the treatment groups were significantly improved versus the control (P < 0.05). The feed conversion ratio was reduced significantly in treatment groups compared to the control (P < 0.05). Compared with the control, alkaline phosphatase (AKP), acid phosphatase (ACP), lysozyme (LZM), catalase (CAT), superoxide dismutase (SOD) activities in the hepatopancreas of the treatment groups were significantly enhanced, and malondialdehyde (MDA) content was reduced significantly (P < 0.05). Compared with the control, the relative expression levels of AKP, ACP, LZM, CAT, SOD, Hsp70, peroxiredoxin-5, Toll, dorsal and relish genes were significantly higher among treatment groups, except for the AKP gene in the LCB1 group and the Hsp70 gene in the LCB1.5 group (P < 0.05). Compared with the control, the relative expression levels of TOR, 4E-BP, eIF4E1α and eIF4E2 genes were significantly enhanced in the LCB1.5 group (P < 0.05). When resistance against Vibrio parahaemolyticus in prawn is considered, higher doses of Bovine lactoferricin show better antibacterial ability. The present study indicated that dietary Bovine lactoferricin could significantly improve the growth performance and improve the antioxidative status of M. rosenbergii. The suitable addition level is 1.5 g/kg. LFcinB has great potential as a new feed additive without the threat of drug resistance.
本研究旨在探讨饲粮添加牛乳铁蛋白（LFcinB）对罗氏沼虾生长性能和非特异性免疫的影响。设计 5 种试验饲粮，分别在基础饲粮中添加 1.0‰（LCB1）、1.5‰（LCB1.5）、2.0‰（LCB2）、2.5‰（LCB2.5）牛乳铁蛋白和不添加牛乳铁蛋白（对照组）。将 600 尾罗氏沼虾随机分为 5 组，每组设 3 个重复，每个重复 40 尾虾，在 15 个水族箱中进行为期 8 周的饲养试验。结果表明：与对照组相比，各试验组罗氏沼虾的末重、增重率、特定生长率和成活率均显著提高（P<0.05）；饲料转化率显著降低（P<0.05）。与对照组相比，各试验组罗氏沼虾肝胰腺碱性磷酸酶（AKP）、酸性磷酸酶（ACP）、溶菌酶（LZM）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活力显著提高，丙二醛（MDA）含量显著降低（P<0.05）。与对照组相比，除 LCB1 组 AKP 基因和 LCB1.5 组 Hsp70 基因外，各试验组 AKP、ACP、LZM、CAT、SOD、Hsp70、过氧化物还原酶-5、Toll、dorsal 和 relish 基因的相对表达量均显著升高（P<0.05）。与对照组相比，LCB1.5 组 TOR、4E-BP、eIF4E1α 和 eIF4E2 基因的相对表达量显著增强（P<0.05）。当考虑对副溶血弧菌的抗性时，较高剂量的牛乳铁蛋白显示出更好的抗菌能力。本研究表明，饲粮添加牛乳铁蛋白可显著提高罗氏沼虾的生长性能，改善其抗氧化状态，适宜添加水平为 1.5 g/kg。LFcinB 作为一种新型饲料添加剂具有很大的潜力，且不存在耐药性的威胁。
