Department of Gastrology, Ankara Training and Research Hospital, Ankara, Turkey.
Department of Rheumatology, Faculty of Medicine Ankara City Hospital, Ankara Yıldırım Beyazıt University, Ankara, Turkey.
Clin Biochem. 2023 Jan;111:81-86. doi: 10.1016/j.clinbiochem.2022.10.012. Epub 2022 Nov 9.
It has been suggested that the deterioration in the antioxidant defense system due to thiols may cause the pro-oxidant/antioxidant imbalance seen in rheumatoid arthritis (RA). This study was conducted to evaluate thiol/disulfide (-SH/-SS) homeostasis in patients with RA compared to healthy controls, and to validate the limited number of studies examining the relationship between Disease Activity Score-28 (DAS28) and thiol parameters.
A total of 100 individuals (mean age: 46.3 ± 12.03) consisting of 86 females and 14 males were included in the RA group, and a total of 100 individuals (mean age: 43.3 ± 10.96 years) consisting of 78 females and 22 males were included in the control group. DAS28 was used to assess RA disease activity. -SH/-SS homeostasis parameters were measured using the automated spectrophotometric method described by Erel and Neselioğlu.
While native thiol (-SH) (p:0.001), total thiol (-SH + -SS) (p < 0.0001) levels and -SH(-SS + -SH) ratio (p: 0.018) were lower in the RA group compared to the healthy controls, disulfide (-SS) level (p: 0.005)), -SS-SH (p: 0.001) and -SS(-SS + -SH) (p: 0.002) ratios were found to be higher. In the control group and the group in remission (defined by DAS28 < 2.6), the median values of -SH (p:0.002) and -SS + -SH (p:0.0008) were found to be significantly higher, and the median value of -SS (p: 0.001) was found to be lower compared to the other DAS28 groups. While a negative correlation was found between DAS28 and -SH (r: -0.243, p: 0.007), a positive correlation was found between DAS28 and -SS (r: 0.316, p: 0.0003), -SS-SH (r:0.229, p: 0.002) and -SS(-SS + -SH) (r: 0.285, p: 0.0009).
The plasma thiol antioxidant pool was decreased in RA compared to healthy controls and those with active disease compared to those in remission.
有人认为，由于硫醇的存在，抗氧化防御系统的恶化可能导致类风湿关节炎（RA）中出现的促氧化剂/抗氧化剂失衡。本研究旨在评估 RA 患者与健康对照组之间的硫醇/二硫键（-SH/-SS）稳态，并验证有限数量的研究检验疾病活动评分 28（DAS28）与硫醇参数之间的关系。
共纳入 100 名个体（平均年龄：46.3±12.03 岁），包括 86 名女性和 14 名男性，纳入 RA 组；共纳入 100 名个体（平均年龄：43.3±10.96 岁），包括 78 名女性和 22 名男性，纳入对照组。使用 DAS28 评估 RA 疾病活动度。采用 Erel 和 Neselioğlu 描述的自动化分光光度法测量 -SH/-SS 稳态参数。
与健康对照组相比，RA 组的内源性巯基（-SH）（p：0.001）、总巯基（-SH+ -SS）（p < 0.0001）水平和 -SH(-SS+ -SH) 比值（p：0.018）较低，而二硫键（-SS）水平（p：0.005）、-SS(-SH）（p：0.001）和 -SS(-SS+ -SH）（p：0.002）比值较高。在对照组和缓解组（定义为 DAS28<2.6）中，-SH（p：0.002）和 -SS+ -SH（p：0.0008）的中位数显著升高，-SS（p：0.001）的中位数降低与其他 DAS28 组相比。DAS28 与 -SH 呈负相关（r：-0.243，p：0.007），而 DAS28 与 -SS（r：0.316，p：0.0003）、-SS(-SH）（r：0.229，p：0.002）和 -SS(-SS+ -SH）（r：0.285，p：0.0009）呈正相关。
与健康对照组和缓解期患者相比，RA 患者的血浆硫醇抗氧化池减少。
