Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jan;263:109499. doi: 10.1016/j.cbpc.2022.109499. Epub 2022 Nov 3.
Lufenuron is an effective benzoylurea insecticide that inhibits the synthesis of chitin and regulates the growth of insects. However, little is known about the effects of lufenuron treatment on the development of Spodoptera frugiperda (J. E. Smith). In this study, we assessed the toxicity of lufenuron on S. frugiperda and evaluated the effects of lufenuron treatment on the growth and development of S. frugiperda. The results showed that lufenuron exhibits high insecticidal activity against S. frugiperda, with the LC value of 0.99 mg L. Lufenuron treatments can significantly prolong the larval developmental duration and reduce the rates of pupation and emergence. To further explore the underlying mechanism of this observation, the expression profiles of the chitin synthase gene (SfCHS) and chitinase gene (SfCHT), two key enzyme genes involved in the molting of S. frugiperda, were determined after exposure to lufenuron for 96 h. The results of qRT-PCR demonstrated that lufenuron treatments can significantly reduce the expression of SfCHT, while the expression of SfCHS remained relatively stable. Furthermore, we found that lufenuron strongly interacted with chitinase (SfCHT) (-10.8 kcal/mol) and chitin synthase (SfCHS) (R1: -9.7 kcal/mol; R2: -10.2 kcal/mol). Our results indicated that lufenuron has significant effects on the development of S. frugiperda that might be attributed to the differential expression of SfCHT and SfCHS.
灭幼脲是一种有效的苯甲酰脲类杀虫剂，它可以抑制几丁质的合成并调节昆虫的生长。然而，人们对灭幼脲处理对美洲棉铃虫（J. E. Smith）发育的影响知之甚少。在本研究中，我们评估了灭幼脲对美洲棉铃虫的毒性，并评估了灭幼脲处理对美洲棉铃虫生长发育的影响。结果表明，灭幼脲对美洲棉铃虫具有很高的杀虫活性，其 LC 值为 0.99mg/L。灭幼脲处理可显著延长幼虫的发育时间，并降低化蛹率和羽化率。为了进一步探讨这一观察结果的潜在机制，我们在暴露于灭幼脲 96 小时后，测定了参与美洲棉铃虫蜕皮的两个关键酶基因（ SfCHS ）和几丁质酶基因（ SfCHT ）的表达谱。qRT-PCR 结果表明，灭幼脲处理可显著降低 SfCHT 的表达，而 SfCHS 的表达则相对稳定。此外，我们发现灭幼脲与几丁质酶（ SfCHT ）（-10.8kcal/mol）和几丁质合成酶（ SfCHS ）（R1：-9.7kcal/mol；R2：-10.2kcal/mol）强烈相互作用。我们的研究结果表明，灭幼脲对美洲棉铃虫的发育有显著影响，这可能归因于 SfCHT 和 SfCHS 的差异表达。
