Gautam Pooja, Ajit Kamal, Das Moitreyi, Taliyan Rajeev, Roy Ramaballav, Banerjee Arnab
Department of Biological Sciences, BITS Pilani, KK Birla, Goa Campus, Goa, India.
Department of Zoology, Goa University, Goa, India.
J Exp Zool A Ecol Integr Physiol. 2023 Mar;339(2):193-209. doi: 10.1002/jez.2671. Epub 2022 Nov 6.
Gonadotropin-releasing hormone (GnRH) is the primary regulator of the mammalian reproductive axis. We investigated the spatiotemporal expression of GnRH splice variants (V1, V2, and V3) and splicing factors (Srsf7, Srsf9, and Tra-2) in the male mice brain. Further, using in silico tools, we predicted protein structure and the reason for the low translational efficiency of V2 and V3. Messenger RNA levels of GnRH variants and splicing factors were quantified using real-time reverse transcription-polymerase chain reaction at different age groups. Our data show that expression of almost all the variants alters with aging in all the brain regions studied; even in comparison to the hypothalamus, several brain areas were found to have higher expression of these variants. Hypothalamic expression of splicing factors such as Srsf7, Srsf9, and Tra-2 also change with aging. Computational studies have translation repressors site on the V3, which probably reduces its translation efficiency. Also, V2 is an intrinsically disordered protein that might have a regulatory or signaling function. In conclusion, this study provides novel crucial information and multiple starting points for future analysis of GnRH splice variants in the brain.
促性腺激素释放激素（GnRH）是哺乳动物生殖轴的主要调节因子。我们研究了GnRH剪接变体（V1、V2和V3）以及剪接因子（Srsf7、Srsf9和Tra-2）在雄性小鼠大脑中的时空表达。此外，我们使用计算机工具预测了蛋白质结构以及V2和V3翻译效率低的原因。通过实时逆转录-聚合酶链反应对不同年龄组的GnRH变体和剪接因子的信使核糖核酸水平进行了定量。我们的数据表明，在所研究的所有脑区中，几乎所有变体的表达都会随着衰老而改变；甚至与下丘脑相比，发现几个脑区中这些变体的表达更高。剪接因子如Srsf7、Srsf9和Tra-2在下丘脑的表达也会随着衰老而变化。计算机研究发现V3上存在翻译抑制位点，这可能会降低其翻译效率。此外，V2是一种内在无序的蛋白质，可能具有调节或信号功能。总之，本研究为未来大脑中GnRH剪接变体的分析提供了新的关键信息和多个切入点。
