Moradi Arash, Aleyasin Seyed Ahmad, Mohammadian Kamal, Alizamir Aida
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Department of Radiation Oncology, Hamadan University of Medical Sciences, Mahdieh center, Hamadan, Iran.
Iran J Biotechnol. 2022 Apr 1;20(2):e3050. doi: 10.30498/ijb.2022.285841.3050. eCollection 2022 Apr.
Intestinal metaplasia (IM) is a benign lesion with no serious concern for patients' health. On the other hand, gastric cancer (GC) is a malignant lesion that has to be differentially diagnosed from benign intestinal metaplasia. Epigenetic modifications have been suggested to play an important role in cancer initiation and development, and they have been investigated as a reliable biomarker tool even for early cancer diagnosis. Whole blood leucocytes (WBC) are potentially the most accessible tissue for cancer early diagnosis, especially for GC, which is hard to diagnose in the early stage.
This study aims to investigate the methylation status of gene CpG island in blood leukocytes of patients with IM and GC compared to normal control groups.
DNA was extracted from the whole blood of 30 IM patients, 30 GC patients, and 34 normal controls samples, and MSRE-PCR was utilized to evaluate the loci methylation status.
Significant hypermethylation of gene CpG has been observed in GC 88.1 % (p < 0.001) and IM 66.0 % (p = 0.03) in comparison to the normal control group 56.8%. A cutoff upper than 84.5 % of hypermethylation is considered as a presence of gastric cancer malignant lesions.
This is the first reported on hypermethylation in CPG in blood leukocytes of patients with GC and IM and establishing a laboratory blood based test that may be useful as a novel biomarker test in the early diagnosis and screaning of GC and IM.
肠化生（IM）是一种良性病变，对患者健康无严重影响。另一方面，胃癌（GC）是一种恶性病变，必须与良性肠化生进行鉴别诊断。表观遗传修饰已被认为在癌症的发生和发展中起重要作用，并且它们已被研究作为一种可靠的生物标志物工具，甚至用于早期癌症诊断。全血白细胞（WBC）可能是癌症早期诊断中最容易获取的组织，特别是对于早期难以诊断的胃癌。
本研究旨在调查与正常对照组相比，IM和GC患者血白细胞中基因CpG岛的甲基化状态。
从30例IM患者、30例GC患者和34例正常对照样本的全血中提取DNA，并利用甲基化敏感限制性内切酶-PCR（MSRE-PCR）评估基因座甲基化状态。
与正常对照组的56.8%相比，在GC中观察到基因CpG的显著高甲基化率为88.1%（p<0.001），在IM中为66.0%（p=0.03）。高于84.5%的高甲基化临界值被认为存在胃癌恶性病变。
这是首次报道GC和IM患者血白细胞中CPG的高甲基化，并建立了一种基于血液的实验室检测方法，该方法可能作为一种新型生物标志物检测方法用于GC和IM的早期诊断和筛查。
