Zhong Wei, Luo Guoliang, Luo Jing, Guo Li
Animal Science and Technology College, Jilin Agricultural Science and Technology University, Jilin, China.
State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun, China.
Front Vet Sci. 2022 Oct 13;9:986388. doi: 10.3389/fvets.2022.986388. eCollection 2022.
The proportion of n-6/n-3 polyunsaturated fatty acid (PUFA) plays an important role in regulating lipid metabolism. This study aimed to investigate the effects of dietary n-6/n-3 PUFA ratios on body fat deposition, tissues fatty acid composition, and gene expression of liver lipid metabolism of silver foxes during the winter fur growth period.
Forty-eight age-matched male silver foxes with similar body weights were randomly divided into four dietary groups for 47 days, which were fed n-6/n-3 PUFA ratio with 3, 18, 41, and 136 experimental diets, respectively.
Dietary n-6/n-3 PUFA ratio did not significantly influence fat deposition parameters except for hepatic fat content. The variation trend of the fatty acid composition of liver, intramuscular fat, and subcutaneous fat in silver fox was directly related to dietary fatty acid content ( < 0.05). With the dietary n-6/n-3 PUFA ratio increasing, the expression of liver fatty acid synthase (FAS) mRNA and peroxisome proliferator-activated receptor (PPAR) mRNA exhibited the trend of first decreasing and then increasing ( < 0.05), whereas L-fatty acid binding protein (L-FABP) mRNA expression showed a gradual increasing trend ( < 0.05).
In summary, silver foxes fed an n-6/n-3 PUFA ratio 18:1 diet (supplementing with 9.38% corn oil and 4.62% soybean oil) was more conducive to lipid decomposition, PUFA transport, and utilization of tissues, thereby meeting it for supplying energy and withstanding the cold.
n-6/n-3多不饱和脂肪酸（PUFA）比例在调节脂质代谢中起重要作用。本研究旨在探讨冬季毛发生长期日粮n-6/n-3 PUFA比例对银狐体脂沉积、组织脂肪酸组成及肝脏脂质代谢基因表达的影响。
将48只年龄匹配、体重相近的雄性银狐随机分为4组，分别饲喂n-6/n-3 PUFA比例为3、18、41和136的实验日粮，为期47天。
日粮n-6/n-3 PUFA比例除对肝脏脂肪含量有显著影响外，对脂肪沉积参数无显著影响。银狐肝脏、肌内脂肪和皮下脂肪的脂肪酸组成变化趋势与日粮脂肪酸含量直接相关（P<0.05）。随着日粮n-6/n-3 PUFA比例升高，肝脏脂肪酸合酶（FAS）mRNA和过氧化物酶体增殖物激活受体（PPAR）mRNA表达呈先降低后升高趋势（P<0.05），而肝脏脂肪酸结合蛋白（L-FABP）mRNA表达呈逐渐升高趋势（P<0.05）。
综上所述，饲喂n-6/n-3 PUFA比例为18:1日粮（添加9.38%玉米油和4.62%大豆油）的银狐更有利于脂质分解、PUFA在组织中的转运和利用，从而满足其供能和抗寒需求。
