Inhibitory effect of carp muscle trypsin inhibitor on mammalian pancreatic proteinases.

Carp muscle trypsin inhibitor showed an inhibitory effect on bovine trypsin, bovine alpha-chymotrypsin and porcine elastase in a non-competitive, competitive and competitive manner, respectively. The inhibitor formed a stable complex with the above proteinases which was not dissociated in the presence of 2-mercaptoethanol and SDS. The true target proteinase for carp muscle trypsin inhibitor, as yet unknown, seems to be an alpha-chymotrypsin- or elastase-like enzyme rather than trypsin, judging from the manner of inhibition.