Kuhla Björn, van Ackern Isabel
Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology 'Oskar Kellner', Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
JDS Commun. 2022 Feb 10;3(2):138-141. doi: 10.3168/jdsc.2021-0185. eCollection 2022 Mar.
Among the endocannabinoids, -arachidonylethanolamide (AEA; anandamide) plays a key role in regulating energy homeostasis and energy intake. Recent studies suggest the existence of a peripheral mechanism by which AEA increases feed intake in the short term and modulates whole-body energy metabolism in dairy cows. Here, we aimed to test the hypothesis that AEA has a long-lasting central effect in increasing feed intake that leads to an increase in milk yield of dairy cows. In the present pilot study, 3 nonpregnant Holstein dairy cows were equipped with an intracerebroventricular (i.c.v.) catheter. Cows were deprived from feed for 2 h and received either no injection or an i.c.v. injection of either 12 μg of AEA or DMSO (control), followed by measurement of feed intake for 10 h and milk yield. Administration of AEA increased 10-h dry matter intake (DMI) by between 1.13 and 2.06 kg, whereas 22-h DMI was only marginally altered. However, compared with the control treatment, AEA reduced daily milk yield by 0.3 to 1.4 L/d in all 3 cows. The results demonstrate that i.c.v. administration of 12 µg of AEA increased 10-h DMI but decreased daily milk yield by a central mechanism.
在内源性大麻素中，花生四烯酸乙醇胺（AEA；花生四烯酸乙醇胺）在调节能量平衡和能量摄入方面发挥着关键作用。最近的研究表明，存在一种外周机制，通过该机制，AEA在短期内增加采食量，并调节奶牛的全身能量代谢。在此，我们旨在验证这一假设：AEA在增加采食量方面具有持久的中枢效应，进而导致奶牛产奶量增加。在本初步研究中，3头未怀孕的荷斯坦奶牛被植入脑室内（i.c.v.）导管。奶牛禁食2小时，然后要么不注射，要么脑室内注射12μg AEA或二甲基亚砜（对照），随后测量10小时的采食量和产奶量。注射AEA使10小时干物质摄入量（DMI）增加了1.13至2.06千克，而22小时DMI仅略有变化。然而，与对照处理相比，AEA使所有3头奶牛的日产奶量降低了0.3至1.4升/天。结果表明，脑室内注射12μg AEA通过中枢机制增加了10小时DMI，但降低了日产奶量。
