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用于新冠病毒诊断的侧向层析芯片中核酸和蛋白质的同步快速检测

Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis.

作者信息

Lin Qiuyuan, Zhang Jin, Liu Liling, Kong Jilie, Fang Xueen

机构信息

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China.

Qingdao International Travel Healthcare Center, Qingdao Customs, Qingdao 266071, P. R. China.

出版信息

ACS Omega. 2022 Oct 21;7(43):38409-38416. doi: 10.1021/acsomega.2c03499. eCollection 2022 Nov 1.

DOI:10.1021/acsomega.2c03499
PMID:36340144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9631717/
Abstract

In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG antibody) with respective detection limits of 1 copy/μL for nucleic acid, 0.85 ng/mL for antigen, and 5.80 ng/mL for IgG within 30 min with high stability and anti-interference ability. The capability of this system in clinical applications was further evaluated using clinical samples, displaying 100% sensitivity and 100% specificity for COVID-19 diagnosis. These findings demonstrate the potential of this method to be used for the detection and subsequent control of pathogens.

摘要

在本研究中，我们报道了一种用于同时检测核酸和蛋白质的快速、便携式且经济的微流控平台。以严重急性呼吸综合征冠状病毒2（SARS-CoV-2）作为检测目标，该微流控芯片能够在30分钟内同时检测SARS-CoV-2核糖核酸（N基因）、抗原（或特异性IgG抗体），核酸的检测限为1拷贝/微升，抗原的检测限为0.85纳克/毫升，IgG的检测限为5.80纳克/毫升，具有高稳定性和抗干扰能力。使用临床样本进一步评估了该系统在临床应用中的能力，对2019冠状病毒病（COVID-19）诊断显示出100%的灵敏度和100%的特异性。这些发现证明了该方法在病原体检测及后续控制方面的应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/98587daccb4d/ao2c03499_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/462b18d64436/ao2c03499_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/aa4b86356685/ao2c03499_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/170d0ae97c80/ao2c03499_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/55ab57f040cb/ao2c03499_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/f16f2c0a24d1/ao2c03499_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/98587daccb4d/ao2c03499_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/462b18d64436/ao2c03499_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/aa4b86356685/ao2c03499_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/170d0ae97c80/ao2c03499_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/55ab57f040cb/ao2c03499_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/f16f2c0a24d1/ao2c03499_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aca5/9631717/98587daccb4d/ao2c03499_0007.jpg

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本文引用的文献

1
Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2.生物扫描：一种基于 CRISPR/dCas9 的侧向流动分析，用于快速、特异性和灵敏检测 SARS-CoV-2。
ACS Synth Biol. 2022 Jan 21;11(1):406-419. doi: 10.1021/acssynbio.1c00499. Epub 2021 Dec 23.
2
Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants.用于基于CRISPR的SARS-CoV-2及新出现变体即时诊断的微创SHERLOCK（miSHERLOCK）
Sci Adv. 2021 Aug 6;7(32). doi: 10.1126/sciadv.abh2944. Print 2021 Aug.
3
Multiplexed and High-Throughput Label-Free Detection of RNA/Spike Protein/IgG/IgM Biomarkers of SARS-CoV-2 Infection Utilizing Nanoplasmonic Biosensors.
利用纳米等离子体生物传感器对新冠病毒感染的RNA/刺突蛋白/IgG/IgM生物标志物进行多重高通量无标记检测。
Anal Chem. 2021 Jun 29;93(25):8754-8763. doi: 10.1021/acs.analchem.0c05300. Epub 2021 Jun 14.
4
Simultaneous detection of small molecules, proteins and microRNAs using single molecule arrays.使用单分子阵列同时检测小分子、蛋白质和微小核糖核酸。
Chem Sci. 2020 Jul 8;11(30):7896-7903. doi: 10.1039/d0sc02552f.
5
A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection.一种用于快速检测新型冠状病毒肺炎的微流控集成侧向流动重组酶聚合酶扩增（MI-IF-RPA）检测方法。
Lab Chip. 2021 May 18;21(10):2019-2026. doi: 10.1039/d0lc01222j.
6
A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis.用于 COVID-19 诊断的快速、准确、可扩展且便携的检测系统。
Nat Commun. 2021 May 18;12(1):2905. doi: 10.1038/s41467-021-23185-x.
7
A smartphone-read ultrasensitive and quantitative saliva test for COVID-19.智能手机读取的超灵敏定量唾液新冠病毒检测。
Sci Adv. 2021 Jan 8;7(2). doi: 10.1126/sciadv.abe3703. Print 2021 Jan.
8
An enhanced isothermal amplification assay for viral detection.一种增强的病毒检测等温扩增检测方法。
Nat Commun. 2020 Nov 20;11(1):5920. doi: 10.1038/s41467-020-19258-y.
9
Target-induced molecular-switch on triple-helix DNA-functionalized carbon nanotubes for simultaneous visual detection of nucleic acids and proteins.基于三螺旋 DNA 功能化碳纳米管的靶标诱导分子开关用于同时可视化检测核酸和蛋白质。
Chem Commun (Camb). 2020 Nov 14;56(88):13657-13660. doi: 10.1039/d0cc05986b. Epub 2020 Oct 16.
10
Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19.超高敏高分辨分析 COVID-19 患者早期血清转换。
Nat Biomed Eng. 2020 Dec;4(12):1180-1187. doi: 10.1038/s41551-020-00611-x. Epub 2020 Sep 18.