Kuzmyn Andriy R, Teunissen Lucas W, Kroese Michiel V, Kant Jet, Venema Sandra, Zuilhof Han
Laboratory of Organic Chemistry, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
Wageningen Bioveterinary Research, Houtribweg 39, 8221 RA Lelystad, The Netherlands.
ACS Omega. 2022 Oct 20;7(43):38371-38379. doi: 10.1021/acsomega.2c03214. eCollection 2022 Nov 1.
This work presents a novel route for creating metal-free antiviral coatings based on polymer brushes synthesized by surface-initiated photoinduced electron transfer-reversible addition-fragmentation chain transfer (SI-PET-RAFT) polymerization, applying eosin Y as a photocatalyst, water as a solvent, and visible light as a driving force. The polymer brushes were synthesized using -[3-(decyldimethyl)-aminopropyl] methacrylamide bromide and carboxybetaine methacrylamide monomers. The chemical composition, thickness, roughness, and wettability of the resulting polymer brush coatings were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), water contact angle measurements, and ellipsometry. The antiviral properties of coatings were investigated by exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and avian influenza viruses, with further measurement of residual viable viral particles. The best performance was obtained with Cu surfaces, with a ca. 20-fold reduction of SARS-Cov-2 and a 50-fold reduction in avian influenza. On the polymer brush-modified surfaces, the number of viable virus particles decreased by about 5-6 times faster for avian flu and about 2-3 times faster for SARS-CoV-2, all compared to unmodified silicon surfaces. Interestingly, no significant differences were obtained between quaternary ammonium brushes and zwitterionic brushes.
这项工作提出了一种基于表面引发光致电子转移-可逆加成-断裂链转移（SI-PET-RAFT）聚合合成的聚合物刷来制备无金属抗病毒涂层的新途径，该方法使用曙红Y作为光催化剂，水作为溶剂，可见光作为驱动力。聚合物刷是使用-[3-(癸基二甲基)氨基丙基]甲基丙烯酰胺溴化物和羧基甜菜碱甲基丙烯酰胺单体合成的。通过X射线光电子能谱（XPS）、原子力显微镜（AFM）、水接触角测量和椭偏仪对所得聚合物刷涂层的化学成分、厚度、粗糙度和润湿性进行了表征。通过暴露于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和禽流感病毒来研究涂层的抗病毒性能，并进一步测量残留的活病毒颗粒。铜表面表现出最佳性能，SARS-CoV-2减少约20倍，禽流感减少50倍。与未改性的硅表面相比，在聚合物刷改性的表面上，禽流感活病毒颗粒数量减少的速度快约5-6倍，SARS-CoV-2减少的速度快约2-3倍。有趣的是，季铵刷和两性离子刷之间没有显著差异。
