Bai Xiulin, Zhou Yujie, Feng Xuping, Tao Mingzhu, Zhang Jinnuo, Deng Shuiguang, Lou Binggan, Yang Guofeng, Wu Qingguan, Yu Li, Yang Yong, He Yong
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Zhuji Agricultural Technology Extension Center, Zhuji, China.
Front Plant Sci. 2022 Oct 19;13:1037774. doi: 10.3389/fpls.2022.1037774. eCollection 2022.
Hyperspectral imaging technique combined with machine learning is a powerful tool for the evaluation of disease phenotype in rice disease-resistant breeding. However, the current studies are almost carried out in the lab environment, which is difficult to apply to the field environment. In this paper, we used visible/near-infrared hyperspectral images to analysis the severity of rice bacterial blight (BB) and proposed a novel disease index construction strategy (NDSCI) for field application. A designed long short-term memory network with attention mechanism could evaluate the BB severity robustly, and the attention block could filter important wavelengths. Best results were obtained based on the fusion of important wavelengths and color features with an accuracy of 0.94. Then, NSDCI was constructed based on the important wavelength and color feature related to BB severity. The correlation coefficient of NDSCI extended to the field data reached -0.84, showing good scalability. This work overcomes the limitations of environmental conditions and sheds new light on the rapid measurement of phenotype in disease-resistant breeding.
高光谱成像技术与机器学习相结合是水稻抗病育种中评估疾病表型的有力工具。然而,目前的研究几乎都是在实验室环境中进行的,难以应用于田间环境。在本文中,我们使用可见/近红外高光谱图像分析水稻白叶枯病(BB)的严重程度,并提出了一种适用于田间应用的新型病害指数构建策略(NDSCI)。一种设计的带有注意力机制的长短期记忆网络能够稳健地评估BB严重程度,并且注意力模块可以筛选出重要波长。基于重要波长和颜色特征的融合获得了最佳结果,准确率为0.94。然后,基于与BB严重程度相关的重要波长和颜色特征构建了NDSCI。扩展到田间数据的NDSCI的相关系数达到-0.84,显示出良好的可扩展性。这项工作克服了环境条件的限制,为抗病育种中表型的快速测量提供了新的思路。