Lynch Tarah, Nandi Tannistha, Jayaprakash Teenus, Gregson Dan, Church Deirdre L
Department of Pathology & Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Research Computing Services, Information Technologies, University of Calgary, Calgary, Alberta, Canada.
J Assoc Med Microbiol Infect Dis Can. 2022 Feb 24;7(1):23-35. doi: 10.3138/jammi-2021-0018. eCollection 2022 Mar.
In 2004-2005, an outbreak of impetigo occurred at a correctional facility during a sentinel outbreak of methicillin- resistant (MRSA) in Alberta, Canada. Next-generation sequencing (NGS) was used to characterize the group A (GAS) isolates and evaluate whether genomic biomarkers could distinguish between those recovered alone and those co-isolated with .
Superficial wound swabs collected from all adults with impetigo during this outbreak were cultured using standard methods. NGS was used to characterize and compare all of the GAS and genomes.
Fifty-three adults were culture positive for GAS, with a subset of specimens also positive for MRSA ( = 5) or methicillin-sensitive ( = 3). Seventeen additional MRSA isolates from this facility from the same time frame (no GAS co-isolates) were also included. All 78 bacterial genomes were analyzed for the presence of known virulence factors, plasmids, and antimicrobial resistance (AMR) genes. Among the GAS isolates were 12 types, the most common being 41.2 ( = 27; 51%). GAS genomes were phylogenetically compared with local and public datasets of invasive and non-invasive isolates. GAS genomes had diverse profiles for virulence factors, plasmids, and AMR genes. Pangenome analysis did not identify horizontally transferred genes in the co-infection versus single infections.
GAS recovered from invasive and non-invasive sources were not genetically distinguishable. Virulence factors, plasmids, and AMR profiles grouped by type, and no genetic changes were identified that predict co-infection or horizontal gene transfer between GAS and .
2004 - 2005年，在加拿大艾伯塔省耐甲氧西林金黄色葡萄球菌（MRSA）的哨兵疫情期间，一所惩教机构发生了脓疱病暴发。采用下一代测序（NGS）对A组（GAS）分离株进行特征分析，并评估基因组生物标志物是否能够区分单独分离出的菌株和与……共同分离出的菌株。
本次疫情期间，从所有患有脓疱病的成年人身上采集的浅表伤口拭子，采用标准方法进行培养。NGS用于对所有GAS和……基因组进行特征分析和比较。
53名成年人GAS培养呈阳性，部分标本MRSA（ = 5）或甲氧西林敏感……（ = 3）也呈阳性。还纳入了同一时期该机构另外17株MRSA分离株（无GAS共同分离株）。对所有78个细菌基因组分析其已知毒力因子、质粒和抗菌药物耐药性（AMR）基因的存在情况。在GAS分离株中有12种……类型，最常见的是41.2（ = 27；51%）。将GAS基因组与侵袭性和非侵袭性分离株的本地及公共数据集进行系统发育比较。GAS基因组在毒力因子、质粒和AMR基因方面具有不同的图谱。泛基因组分析未在合并感染与单一感染中鉴定出水平转移基因。
从侵袭性和非侵袭性来源分离出的GAS在基因上无法区分。毒力因子、质粒和AMR图谱按……类型分组，未发现预测GAS与……之间合并感染或水平基因转移的基因变化。
