USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Mycotoxin Prevention and Applied Microbiology Unit, 1815 N. University St., Peoria, IL 61604, USA.
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
Fungal Genet Biol. 2022 Nov;163:103749. doi: 10.1016/j.fgb.2022.103749. Epub 2022 Oct 29.
The Fusarium verticillioides SKC1 gene driver is transmitted to offspring in a biased manner through spore killing. The mechanism that allows SKC1 to kill non-SKC1 offspring while sparing others is poorly understood. Here we report that gene drive by SKC1 is dependent on SKC1's competing allele. We propose that SKC1's competing allele influences the ability of a genome defense process to detect SKC1, and we provide evidence that this genome defense process is meiotic silencing by unpaired DNA (MSUD). Our findings suggest that the successful deployment of gene drivers to control pathogenic fungi will require researchers to consider how competing alleles influence the ability of gene drivers to be detected by genome defense processes.
镰刀菌 verticillioides SKC1 基因驱动器通过孢子致死以偏向的方式传递给后代。SKC1 杀死非 SKC1 后代而保留其他后代的机制尚不清楚。在这里，我们报告说，SKC1 的基因驱动依赖于 SKC1 的竞争等位基因。我们提出，SKC1 的竞争等位基因影响基因组防御过程检测 SKC1 的能力，并且我们提供了证据表明，这种基因组防御过程是减数分裂中未配对 DNA 的沉默（MSUD）。我们的研究结果表明，成功部署基因驱动器来控制致病真菌将要求研究人员考虑竞争等位基因如何影响基因驱动器被基因组防御过程检测的能力。
