MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China.
MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China.
Fish Shellfish Immunol. 2022 Dec;131:729-735. doi: 10.1016/j.fsi.2022.10.056. Epub 2022 Oct 29.
Lysozyme is relevant to the innate immune system as a vital protein for crustaceans. In the present study, we cloned and characterized a novel c-type lysozyme gene (LvLYZ) from the Pacific white shrimp (Litopenaeus vannamei). The obtained full-length cDNA of LvLYZ was 990 bp and contained an open reading frame of 693 bp. Its deduced amino acid sequence consisted of 230 amino acids (aa) with a 17 aa signal peptide at the N-terminal and 130 aa functional domains. The multiple sequence alignment (MSA) indicated that the typical active sites in LvLYZ were similarly conserved as c-type lysozymes from other species. The transcription of LvLYZ appeared in all detected tissues and had relatively higher expression levels in hemocytes, hepatopancreas, gill and intestine. The mRNA expression profiles of LvLYZ were up-regulated in hemocyte and hepatopancreas post the stimulation of Vibrio parahaemolyticus or white spot syndrome virus (WSSV), respectively. The recombinant protein of LvLYZ (rLvLYZ) exhibited antibacterial activities against various microbes, including Escherichia coli, Vibrio splendidus, Micrococcaus luteus, Vibrio parahaemolyticus and Staphylococcus aureus. These results indicated that LvLYZ could cope with bacteria in L. vannamei and may play a significant role in immune response against invading pathogens.
溶菌酶与甲壳动物的先天免疫系统有关，是一种重要的蛋白质。在本研究中，我们从凡纳滨对虾（Litopenaeus vannamei）中克隆并鉴定了一种新型的 c 型溶菌酶基因（LvLYZ）。获得的 LvLYZ 全长 cDNA 为 990 bp，包含一个 693 bp 的开放阅读框。其推导的氨基酸序列由 230 个氨基酸（aa）组成，N 端有 17 个 aa 的信号肽和 130 个 aa 的功能结构域。多重序列比对（MSA）表明，LvLYZ 中的典型活性位点与其他物种的 c 型溶菌酶相似。LvLYZ 的转录在所有检测到的组织中均有出现，在血细胞、肝胰腺、鳃和肠中具有相对较高的表达水平。LvLYZ 的 mRNA 表达谱在血细胞和肝胰腺中分别受到副溶血弧菌或白斑综合征病毒（WSSV）刺激后上调。LvLYZ 的重组蛋白（rLvLYZ）对多种微生物具有抗菌活性，包括大肠杆菌、灿烂弧菌、藤黄微球菌、副溶血弧菌和金黄色葡萄球菌。这些结果表明，LvLYZ 可以应对凡纳滨对虾体内的细菌，可能在抵御入侵病原体的免疫反应中发挥重要作用。
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