Duke University Medical Center, Durham, NC, USA.
University of Florida, Gainesville, FL, USA.
J Cardiovasc Transl Res. 2023 Jun;16(3):748-750. doi: 10.1007/s12265-022-10337-7. Epub 2022 Nov 7.
The porcine intra-abdominal heterotopic heart transplantation model allows for the assessment of immunologic effects on cardiac transplantation without relying on the allograft to maintain hemodynamic support for the animal. Historically, allograft function and histology is monitored by physical exam, echocardiogram evaluation, percutaneous core biopsy, and open biopsy. We performed transvenous endomyocardial biopsies in three pigs that had undergone heterotopic heart implantation. We describe the procedure to be feasible and reproducible, and that histologic results from these biopsies correlated with those from corresponding tissue collected by surgical dissection at the time of allograft explantation. The ability to perform endomyocardial biopsies in the heterotopic heart transplantation model allows for serial non-invasive monitoring of allograft histology.
猪腹腔异位心脏移植模型允许评估免疫效应对心脏移植的影响，而无需依赖同种异体移植物维持动物的血液动力学支持。从历史上看，同种异体移植物的功能和组织学通过体格检查、超声心动图评估、经皮芯活检和开放活检来监测。我们对 3 只接受异位心脏植入的猪进行了经静脉心内膜心肌活检。我们描述了该程序是可行且可重复的，并且这些活检的组织学结果与同种异体移植物取出时通过手术解剖收集的相应组织的结果相关。在异位心脏移植模型中进行心内膜心肌活检的能力允许对同种异体移植物组织学进行连续的非侵入性监测。
