Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou 350002, China.
Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou 350014, China.
Toxicol In Vitro. 2023 Feb;86:105516. doi: 10.1016/j.tiv.2022.105516. Epub 2022 Nov 5.
Electronic cigarette (e-Cig) has been promoted as a safer alternative to traditional cigarette (t-Cig) recently. However, there are limited scientific data on the potential health effects of e-Cig use. In this study, we evaluated the cytotoxicities of e-Cig and t-Cig condensate solutions (e-CigCS and t-CigCS) on human bronchial epithelial cells (16HBE cells) in vitro, and employed the exosome proteomic technique to systematically assess the effects of e-CigCS and t-CigCS on 16HBE cells. Cytotoxicity assay showed 16HBE cells were more sensitive to t-CigCS than e-CigCS. Proteomic analysis demonstrated that there are 431 differential expressed exosomal proteins (DEEPs) in test groups compared to the control air group (P-value<0.05) and t-CigCS has a greater influence than e-CigCS on exosomal protein expression. Bioinformatic analysis showed the DEEPs from the t-Cig group were significantly enriched in pathways in cancer while tobacco-flavored e-Cig (e-Cig) and menthol-flavored e-Cig (e-Cig) groups were not. Further validations of some DEEPs, such as NF-κB p65, Sulfiredoxin-1(SRXN1) and Thioredoxin-interacting protein (TXNIP), were carried out using immunoblot and Real-time PCR analysis, showing that t-Cig may have a greater influence than e-Cig on tumor development and metastasis. Taken together, the finding reported here strongly support our hypothesis that electronic cigarettes are significantly less toxic compared with traditional cigarette.
电子烟（e-Cig）最近被宣传为传统香烟（t-Cig）的更安全替代品。然而，关于电子烟使用的潜在健康影响的科学数据有限。在这项研究中，我们评估了电子香烟和传统香烟冷凝物溶液（e-CigCS 和 t-CigCS）对体外人支气管上皮细胞（16HBE 细胞）的细胞毒性，并采用外泌体蛋白质组学技术系统评估 e-CigCS 和 t-CigCS 对 16HBE 细胞的影响。细胞毒性测定表明，16HBE 细胞对 t-CigCS 的敏感性高于 e-CigCS。蛋白质组学分析表明，与对照空气组相比，试验组中有 431 个差异表达的外泌体蛋白（DEEP）（P 值<0.05），并且 t-CigCS 对外泌体蛋白表达的影响大于 e-CigCS。生物信息学分析表明，来自 t-Cig 组的 DEEPs 在癌症途径中显著富集，而烟草味电子烟（e-Cig）和薄荷醇味电子烟（e-Cig）组则没有。使用免疫印迹和实时 PCR 分析进一步验证了一些 DEEPs，如 NF-κB p65、Sulfiredoxin-1（SRXN1）和 Thioredoxin-interacting protein（TXNIP），结果表明 t-Cig 对肿瘤发展和转移的影响可能大于 e-Cig。综上所述，这里报道的发现有力地支持了我们的假设，即电子烟的毒性明显低于传统香烟。
