Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China.
Optics Valley Laboratory, 430074, Wuhan, Hubei, China.
Commun Biol. 2022 Nov 8;5(1):1200. doi: 10.1038/s42003-022-04159-1.
The development of the neuromuscular system, including muscle growth and intramuscular neural development, in addition to central nervous system maturation, determines motor ability improvement. Motor development occurs asynchronously from cephalic to caudal. However, whether the structural development of different muscles is heterochronic is unclear. Here, based on the characteristics of motor behavior in postnatal mice, we examined the 3D structural features of the neuromuscular system in different muscles by combining tissue clearing with optical imaging techniques. Quantitative analyses of the structural data and related mRNA expression revealed that there was continued myofiber hyperplasia of the forelimb and hindlimb muscles until around postnatal day 3 (P3) and P6, respectively, as well as continued axonal arborization and neuromuscular junction formation until around P3 and P9, respectively; feature alterations of the cervical muscle ended at birth. Such structural heterochrony of muscles in different body parts corresponds to their motor function. Structural data on the neuromuscular system of neonatal muscles provide a 3D perspective in the understanding of the structural status during motor development.
神经系统的发育，包括肌肉生长和肌内神经发育，以及中枢神经系统的成熟，决定了运动能力的提高。运动发育是从头向尾异步发生的。然而，不同肌肉的结构发育是否存在异时性尚不清楚。在这里，我们基于出生后小鼠的运动行为特征，通过结合组织透明化和光学成像技术，检查了不同肌肉的神经肌肉系统的 3D 结构特征。对结构数据和相关 mRNA 表达的定量分析表明，前肢和后肢肌肉的肌纤维增生持续到出生后第 3 天（P3）和第 6 天（P6），轴突分支和神经肌肉接头的形成持续到 P3 和 P9 左右；颈部肌肉的特征改变发生在出生时。不同身体部位肌肉的这种结构异时性与它们的运动功能相对应。新生儿肌肉神经肌肉系统的结构数据提供了对运动发育过程中结构状态的 3D 视角。
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