Department of Pharmaceutical Sciences, University of California, Irvine, CA, USA.
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.
Nat Rev Genet. 2023 Mar;24(3):178-196. doi: 10.1038/s41576-022-00546-w. Epub 2022 Nov 8.
RNA is a key regulator of almost every cellular process, and the structures adopted by RNA molecules are thought to be central to their functions. The recent fast-paced evolution of high-throughput sequencing-based RNA structure mapping methods has enabled the rapid in vivo structural interrogation of entire cellular transcriptomes. Collectively, these studies are shedding new light on the long underestimated complexity of the structural organization of the transcriptome - the RNA structurome. Moreover, recent analyses are challenging the view that the RNA structurome is a static entity by revealing how RNA molecules establish intricate networks of alternative intramolecular and intermolecular interactions and that these ensembles of RNA structures are dynamically regulated to finely tune RNA functions in living cells. This new understanding of how RNA can shape cell phenotypes has important implications for the development of RNA-targeted therapeutic strategies.
RNA 是几乎每个细胞过程的关键调节剂，并且 RNA 分子采用的结构被认为是其功能的核心。基于高通量测序的 RNA 结构图谱方法的快速发展，使得对整个细胞转录组的快速体内结构研究成为可能。总的来说，这些研究揭示了转录组（RNA 结构组）结构组织的长期被低估的复杂性，为其提供了新的视角。此外，最近的分析通过揭示 RNA 分子如何建立复杂的分子内和分子间替代相互作用网络，以及这些 RNA 结构的组合如何动态调节以精细调整活细胞中 RNA 功能，对 RNA 结构组是静态实体的观点提出了挑战。这种对 RNA 如何塑造细胞表型的新理解，对于开发针对 RNA 的治疗策略具有重要意义。
