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植物 U2 小核核糖核蛋白 B″基因家族的系统发育分析和应激反应。

Phylogenetic analysis and stress response of the plant U2 small nuclear ribonucleoprotein B″ gene family.

机构信息

School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China.

National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

出版信息

BMC Genomics. 2022 Nov 8;23(1):744. doi: 10.1186/s12864-022-08956-0.

DOI:10.1186/s12864-022-08956-0
PMID:36348279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9644473/
Abstract

BACKGROUND

Alternative splicing (AS) is an important channel for gene expression regulation and protein diversification, in addition to a major reason for the considerable differences in the number of genes and proteins in eukaryotes. In plants, U2 small nuclear ribonucleoprotein B″ (U2B″), a component of splicing complex U2 snRNP, plays an important role in AS. Currently, few studies have investigated plant U2B″, and its mechanism remains unclear.

RESULT

Phylogenetic analysis, including gene and protein structures, revealed that U2B″ is highly conserved in plants and typically contains two RNA recognition motifs. Subcellular localisation showed that OsU2B″ is located in the nucleus and cytoplasm, indicating that it has broad functions throughout the cell. Elemental analysis of the promoter region showed that it responded to numerous external stimuli, including hormones, stress, and light. Subsequent qPCR experiments examining response to stress (cold, salt, drought, and heavy metal cadmium) corroborated the findings. The prediction results of protein-protein interactions showed that its function is largely through a single pathway, mainly through interaction with snRNP proteins.

CONCLUSION

U2B″ is highly conserved in the plant kingdom, functions in the nucleus and cytoplasm, and participates in a wide range of processes in plant growth and development.

摘要

背景

选择性剪接（AS）是基因表达调控和蛋白质多样化的重要途径，也是真核生物中外显子和蛋白质数量差异较大的主要原因之一。在植物中，剪接复合物 U2 snRNP 的组成部分 U2 小核核糖核蛋白 B″（U2B″）在 AS 中起着重要作用。目前，对植物 U2B″的研究较少，其机制尚不清楚。

结果

系统发育分析，包括基因和蛋白质结构，表明 U2B″在植物中高度保守，通常包含两个 RNA 识别基序。亚细胞定位表明 OsU2B″位于细胞核和细胞质中，表明其在整个细胞中具有广泛的功能。启动子区域的元素分析表明，它对多种外部刺激（激素、胁迫和光）有反应。随后对冷、盐、干旱和重金属镉胁迫的 qPCR 实验验证了这一发现。蛋白质-蛋白质相互作用的预测结果表明，其功能主要通过单一途径实现，主要通过与 snRNP 蛋白的相互作用。

结论

U2B″在植物界高度保守，在细胞核和细胞质中发挥作用，参与植物生长发育的广泛过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/66b57493d7b6/12864_2022_8956_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/37871ff4c26e/12864_2022_8956_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/3a1b94e9e97e/12864_2022_8956_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/66b57493d7b6/12864_2022_8956_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/37871ff4c26e/12864_2022_8956_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/5b62b3fe3c9f/12864_2022_8956_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/68e0559dc147/12864_2022_8956_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/1272dbef5d40/12864_2022_8956_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/eb64d766763c/12864_2022_8956_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/a3a809cf4a52/12864_2022_8956_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/3a1b94e9e97e/12864_2022_8956_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/092c/9644473/66b57493d7b6/12864_2022_8956_Fig8_HTML.jpg

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