Deng Li-Jia, Jia Meng, Luo Si-Yu, Li Feng-Zeng, Fang Sheng
Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
Clin Cosmet Investig Dermatol. 2022 Nov 2;15:2353-2361. doi: 10.2147/CCID.S389551. eCollection 2022.
Basal cell carcinoma (BCC) is the most common type of cancer with a growing incidence rate over recent decades. The increasing economic burden and incidence of BCC have generated major interest in developing targeted therapies for this disease. The critical role of the Hedgehog (Hh) pathway in the pathogenesis of BCC has become evidently demonstrated. The purpose of this study was to observe the expression of PTCH1 and Gli1 in BCC and further evaluate their relationship with clinicopathological features.
This retrospective study included 84 patients with BCC. Information of 84 patients with pathologically diagnosed BCC (including location, sex, tumor size, pathological type, and depth of invasion) were collected, and tissue paraffin blocks were collected for immunohistochemical staining. Western blot analysis for PTCH1 and Gli1 were also performed. The staining intensity and percentage of stained cells were expressed as a histochemical score (HSCORE).
PTCH1 and Gli1 were overexpressed in BCC compared with adjacent normal epidermis. Our study found that the expression of PTCH1 and Gli1 in BCC in exposed sites was significantly higher than in non-exposed sites. Moreover, no significant difference was observed in sex, Breslow thickness, tumor size or pathological type (P>0.05).
PTCH1 and Gli1 were overexpressed in BCC. Higher PTCH1 and Gli1 expression were in exposed sites lesions. Our study suggests that UV radiation may be associated with aberrant activation of the Hh-PTCH1-Gli1 intercellular signaling pathway in BCC. The molecular mechanism of UV-related PTCH1 and Gli1 differential expression deserves more rigorous research in the future.
基底细胞癌（BCC）是最常见的癌症类型，近几十年来发病率不断上升。BCC日益增加的经济负担和发病率引发了人们对开发针对该疾病的靶向治疗方法的极大兴趣。刺猬信号通路（Hh）在BCC发病机制中的关键作用已得到明确证实。本研究的目的是观察PTCH1和Gli1在BCC中的表达，并进一步评估它们与临床病理特征的关系。
这项回顾性研究纳入了84例BCC患者。收集了84例经病理诊断为BCC患者的信息（包括部位、性别、肿瘤大小、病理类型和浸润深度），并收集组织石蜡块进行免疫组化染色。还对PTCH1和Gli1进行了蛋白质印迹分析。染色强度和染色细胞百分比以组织化学评分（HSCORE）表示。
与相邻正常表皮相比，PTCH1和Gli1在BCC中过表达。我们的研究发现，暴露部位BCC中PTCH1和Gli1的表达明显高于非暴露部位。此外，在性别、Breslow厚度、肿瘤大小或病理类型方面未观察到显著差异（P>0.05）。
PTCH1和Gli1在BCC中过表达。暴露部位病变中PTCH1和Gli1表达较高。我们的研究表明，紫外线辐射可能与BCC中Hh-PTCH1-Gli1细胞间信号通路的异常激活有关。紫外线相关的PTCH1和Gli1差异表达的分子机制在未来值得更深入的研究。
