Departments of Trauma Orthopedics, Ningbo No. 6 Hospital, China.
Department of Neurology, The Affiliated Hospital of Medical School, Ningbo University, China.
Tissue Cell. 2022 Dec;79:101967. doi: 10.1016/j.tice.2022.101967. Epub 2022 Oct 31.
The proliferation and migration of Schwann cells is pivotal to peripheral nerve injury (PNI) repair. Recent studies have revealed that Ginkgetin has neuroprotective effects. Hence, we focused on identifying whether Ginkgetin could regulate the proliferation and migration of Schwann cells, thereby contributing to the repair of PNI. Rat Schwann cells RSC96 were treated with different concentrations of Ginkgetin. Short hairpin RNA targeting phosphatidylinositol glycan anchor biosynthesis class F (shPIGF) was employed to investigate the effects of PIGF on Ginkgetin-induced RSC96 cells. Viability of RSC96 cells was estimated via cell counting kit-8 (CCK-8) assay and proliferation of the cells was assessed by 5-ethynyl-2'-deoxyuridine (EdU) assay. Migration was estimated via wound healing assay and invasion was evaluated through transwell assay. Western blot was employed to test the expressions of PIGF, protein-38 (p38), and phosphorylated p-38 (p-p38). Ginkgetin (50 or 100 μg/ml) increased the viability, proliferation, migration, and invasion of RSC96 cells, up-regulated PIGF expression and raised the ratio of p-p38/p38, which were all reversed by PIGF silencing. Ginkgetin promotes proliferation, migration, and invasion of Schwann cells via PIGF/p38 MAPK signaling pathway.
雪中金具有神经保护作用。因此，我们专注于确定雪中金是否可以调节许旺细胞的增殖和迁移，从而有助于周围神经损伤（PNI）的修复。用不同浓度的雪中金处理大鼠许旺细胞 RSC96。使用针对磷脂酰肌醇聚糖锚生物合成类 F（shPIGF）的短发夹 RNA 来研究 PIGF 对雪中金诱导的 RSC96 细胞的影响。通过细胞计数试剂盒-8（CCK-8）测定 RSC96 细胞的活力，通过 5-乙炔基-2'-脱氧尿苷（EdU）测定细胞的增殖。通过划痕愈合测定法评估迁移，通过 Transwell 测定法评估侵袭。通过蛋白质印迹法测试 PIGF、蛋白激酶 38（p38）和磷酸化 p38（p-p38）的表达。雪中金（50 或 100μg/ml）增加了 RSC96 细胞的活力、增殖、迁移和侵袭，上调了 PIGF 的表达，并提高了 p-p38/p38 的比值，这些都被 PIGF 沉默所逆转。雪中金通过 PIGF/p38 MAPK 信号通路促进许旺细胞的增殖、迁移和侵袭。
