Mironov A S, Rozinov M N, Kozlov Iu I, Sukhodolets V V, Rebentish B A
Genetika. 1978 Sep;14(9):1521-9.
A specialized phage lambda ddeo carrying the deo operon of Escherichia coli is analyzed by exposing the DNA to the specific restriction endonucleases EcoRI and BamHI. Using the lambda ddeo DNA fragment, obtained by digestion with BamHI and plasmid RSF2124 as a vehicle, the hybrid plasmid pAM1 carrying all the genes of the deo operon is constructed and cloned in E. coli cells. It is shown that the activity of thymidine phosphorylase in the strain AM061, which contains hybrid plasmid pAM1 is 30-fold greater than that in strains of E. coli with chromosomal localization of the deo operon.
通过将携带大肠杆菌deo操纵子的特异性噬菌体λddeo的DNA暴露于特异性限制性核酸内切酶EcoRI和BamHI来进行分析。使用经BamHI消化获得的λddeo DNA片段以及质粒RSF2124作为载体，构建携带deo操纵子所有基因的杂交质粒pAM1，并将其克隆到大肠杆菌细胞中。结果表明，含有杂交质粒pAM1的AM061菌株中胸苷磷酸化酶的活性比deo操纵子定位于染色体的大肠杆菌菌株中的活性高30倍。
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