Young I G, Poulis M I
Gene. 1978 Oct;4(2):175-9. doi: 10.1016/0378-1119(78)90029-x.
The transfer properties of five cloning vectors derived from ColE1 were studied. Two of the vectors (pSF2124 and pGM706) behaved like wild type ColE1 in that they could be transferred efficiently in the presence of the conjugative plasmid F. The mobilization of the remaining three vectors (pMB9, PBR313 and pBR322) by F was barely detectable. The transfer defect in pBR313 and pBR322 could be complemented by ColK when R64drd11, but not F, was used as the conjugative plasmid. The transferred plasmids could be recovered unchanged from recipients. Conjugal transfer is a potentially useful technique for screening hybrid plasmids in low-risk cloning experiments involving poorly transformable strains.
对源自ColE1的五种克隆载体的转移特性进行了研究。其中两种载体（pSF2124和pGM706）的表现与野生型ColE1相似，即在存在接合质粒F的情况下它们能够高效转移。其余三种载体（pMB9、PBR313和pBR322）由F介导的转移几乎检测不到。当使用R64drd11而非F作为接合质粒时，ColK可弥补pBR313和pBR322的转移缺陷。转移的质粒可完整地从受体中回收。在涉及难以转化的菌株的低风险克隆实验中，接合转移是筛选杂交质粒的一种潜在有用技术。
