Camacho Miladis I, García Janet M, Roget Dianelis, Ferrer Armando, Wieme Anneleen D, Vandamme Peter, Rodríguez Suyén, Llauradó Gabriel, Lescaylle Yaneisy, Peña Leonor, Bonne Javier, Meriño Thais-Len
Centro de Estudios de Biotecnología Industrial (CEBI), Faculty of Natural and Exact Sciences, University of Oriente, Patricio Lumumba, 90500, Santiago de Cuba, Cuba.
Cayo Naranjo Dolphinarium, UEB Marina Gaviota Oriente, Naranjo Bay, Km 58 Guardalavaca Highway, Rafael Freyre, Holguin, Cuba.
Curr Microbiol. 2022 Nov 9;79(12):398. doi: 10.1007/s00284-022-03090-2.
A bacterium strain isolated from freshwater sediment of San Pablo river of Santiago de Cuba, Cuba was identified as a Bacillus sp. by Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry. A 16S rRNA gene analysis showed that the isolate A3 belongs to the operational group Bacillus amyloliquefaciens, while the phylogenetic analysis of the gyrA gene sequence grouped it within B. amyloliquefaciens subsp. plantarum cluster, referred now as Bacillus velezensis. In vitro antibacterial studies demonstrated the capacity of the isolate A3 to produce bioactive metabolites against Bacillus subtilis ATCC 11,778, Bacillus cereus ATCC 6633, and Staphylococcus aureus ATCC 25,923 by cross-streak, overlay, and microdilution methods. The strain also showed a high potential against the multidrug-resistant Staphylococcus aureus ATCC 700,699, ATCC 29,213, and ATCC 6538. At pH 8 and 96 h in the medium 2 of A3 culture conditions, the produced metabolites with antibacterial potential were enhanced. Some alterations in the morphology of the phytopathogens Aspergillus niger ATCC 9642, Alternaria alternata CECT 2662, and Fusarium solani CCEBI 3094 were induced by the cell-free supernatant of B. velezensis A3. A preliminary study of the nature of the bioactive compounds produced by the strain A3 showed the presence of both lipids and peptides in the culture. Those results highlight B. velezensis A3 as a promissory bacterium capable to produce bioactive metabolites with antibacterial and antifungal properties against pathogens.
从古巴圣地亚哥德古巴圣巴勃罗河淡水沉积物中分离出的一株细菌，通过基质辅助激光解吸/电离飞行时间质谱法鉴定为芽孢杆菌属。16S rRNA基因分析表明，分离株A3属于解淀粉芽孢杆菌操作组，而gyrA基因序列的系统发育分析将其归入解淀粉芽孢杆菌植物亚种簇，现称为贝莱斯芽孢杆菌。体外抗菌研究表明，分离株A3能够通过交叉划线、覆盖和平板微量稀释法产生针对枯草芽孢杆菌ATCC 11778、蜡样芽孢杆菌ATCC 6633和金黄色葡萄球菌ATCC 25923的生物活性代谢产物。该菌株对多重耐药金黄色葡萄球菌ATCC 700699、ATCC 29213和ATCC 6538也显示出很高的抗菌潜力。在A3培养条件的培养基2中，pH值为8且培养96小时时，产生的具有抗菌潜力的代谢产物有所增加。贝莱斯芽孢杆菌A3的无细胞上清液诱导了植物病原菌黑曲霉ATCC 9642、链格孢菌CECT 2662和茄类镰刀菌CCEBI 3094的形态发生一些变化。对菌株A3产生的生物活性化合物性质的初步研究表明，培养物中同时存在脂质和肽。这些结果突出了贝莱斯芽孢杆菌A3作为一种有前景的细菌，能够产生对病原体具有抗菌和抗真菌特性的生物活性代谢产物。
