Erben Jakub, Jirkovec Radek, Kalous Tomas, Klicova Marketa, Chvojka Jiri
Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, 461 17 Liberec, Czech Republic.
Bioengineering (Basel). 2022 Nov 7;9(11):660. doi: 10.3390/bioengineering9110660.
This study presents the advantages of combining three-dimensional biodegradable scaffolds with the injection bioprinting of hydrogels. This combination takes advantage of the synergic effect of the properties of the various components, namely the very favorable mechanical and structural properties of fiber scaffolds fabricated from polycaprolactone and the targeted injection of a hydrogel cell suspension with a high degree of hydrophilicity. These properties exert a very positive impact in terms of promoting inner cell proliferation and the ability to create compact tissue. The scaffolds were composed of a mixture of microfibers produced via meltblown technology that ensured both an optimal three-dimensional porous structure and sufficient mechanical properties, and electrospun nanofibers that allowed for good cell adhesion. The scaffolds were suitable for combination with injection bioprinting thanks to their mechanical properties, i.e., only one nanofibrous scaffold became deformed during the injection process. A computer numerical-control manipulator featuring a heated printhead that allowed for the exact dosing of the hydrogel cell suspension into the scaffolds was used for the injection bioprinting. The hyaluronan hydrogel created a favorable hydrophilic ambiance following the filling of the fiber structure. Preliminary in vitro testing proved the high potential of this combination with respect to the field of bone tissue engineering. The ideal structural and mechanical properties of the tested material allowed osteoblasts to proliferate into the inner structure of the sample. Further, the tests demonstrated the significant contribution of printed hydrogel-cell suspension to the cell proliferation rate. Thus, the study led to the identification of a suitable hydrogel for osteoblasts.
本研究展示了将三维可生物降解支架与水凝胶注射生物打印相结合的优势。这种结合利用了各种成分特性的协同效应，即由聚己内酯制成的纤维支架具有非常良好的机械和结构性能，以及靶向注射具有高度亲水性的水凝胶细胞悬液。这些特性在促进内部细胞增殖和形成致密组织的能力方面产生了非常积极的影响。支架由通过熔喷技术生产的微纤维混合物组成，该混合物确保了最佳的三维多孔结构和足够的机械性能，以及允许良好细胞粘附的电纺纳米纤维。由于其机械性能，这些支架适合与注射生物打印相结合，即在注射过程中只有一个纳米纤维支架发生变形。使用具有加热打印头的计算机数控操纵器，该打印头允许将水凝胶细胞悬液精确计量到支架中进行注射生物打印。透明质酸水凝胶在填充纤维结构后营造了有利的亲水环境。初步体外测试证明了这种结合在骨组织工程领域具有很高的潜力。测试材料理想的结构和机械性能使成骨细胞能够增殖到样品的内部结构中。此外，测试表明打印的水凝胶细胞悬液对细胞增殖率有显著贡献。因此，该研究确定了一种适合成骨细胞的水凝胶。
