Nogueira-Lima Suzana Helena Campelo, Gomes Paulo Wender P, Navegantes-Lima Kely C, Reis José Diogo E, Carvalho Alice Rhelly Veloso, Pamplona Sônia das Graças Santa R, Muribeca Abraão de Jesus B, da Silva Milton N, Monteiro Marta C, E Silva Consuelo Yumiko Yoshioka
Institute of Health Sciences, Postgraduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém 66075-110, Brazil.
Collaborative Mass Spectrometry Innovation Center, University of California San Diego, La Jolla, San Diego, CA 92093, USA.
Metabolites. 2022 Nov 8;12(11):1083. doi: 10.3390/metabo12111083.
(Benth.) A.M.G.Azevedo & R.A.Camargo (Fabaceae) is an herbaceous plant distributed in the Brazilian Amazon, and it is called "raiz do sol" (sun roots). On Marajó Island, quilombola communities use its prepared roots to treat skin diseases commonly caused by fungi, viruses, and bacteria. Thus, in this study, the extract, and its fractions from roots were used to perform in vitro cytotoxic and antibacterial assays against strains. Thereafter, liquid chromatography-mass spectrometry (LC-MS) was used for the metabolite annotation process. The ethanolic extract of roots show significant bactericidal activity against with IC 82 μg.mL and a selectivity index (SI) of 21.35. Furthermore, the SREFr2 and SREFr3 fractions show a potent bactericidal activity, i.e., MIC of 46.8 μg.mL for both, and MBC of 375 and 93.7 μg.mL, respectively. As showcased, SREFr3 shows safe and effective antibacterial activity mainly in respect to the excellent selectivity index (SI = 82.06). On the other hand, SREFr2 shows low selectivity (SI = 6.8), which characterizes it as not safe for therapeutic use. Otherwise, due to a limited amount of reference MS spectra in public libraries, up to now, it was not possible to perform a complete metabolite annotation. Despite that, our antibacterial results for SREFr3 and correlated substructures of amino acid derivatives show that the roots of are a natural source of specialized metabolites, which can be isolated in the future, and then used as a support for further bio-guided research, as well as natural drug development.
（本特）A.M.G.阿泽维多和R.A.卡马戈（豆科）是一种分布于巴西亚马逊地区的草本植物，它被称为“太阳根”。在马拉若岛，基隆波拉社区使用其处理过的根来治疗通常由真菌、病毒和细菌引起的皮肤病。因此，在本研究中，该植物根的提取物及其馏分被用于对菌株进行体外细胞毒性和抗菌试验。此后，液相色谱 - 质谱联用（LC - MS）用于代谢物注释过程。该植物根的乙醇提取物对[具体菌株]显示出显著的杀菌活性，IC为82μg.mL，选择性指数（SI）为21.35。此外，SREFr2和SREFr3馏分显示出强效杀菌活性，即两者的MIC均为46.8μg.mL，MBC分别为375和93.7μg.mL。如图所示，SREFr3主要因其优异的选择性指数（SI = 82.06）而显示出安全有效的抗菌活性。另一方面，SREFr2显示出低选择性（SI = 6.8），这表明其在治疗用途上不安全。否则，由于公共库中参考质谱图数量有限，到目前为止，无法进行完整的代谢物注释。尽管如此，我们对SREFr3的抗菌结果以及氨基酸衍生物的相关亚结构表明，该植物的根是专门代谢物的天然来源，未来可以分离出来，然后用作进一步生物导向研究以及天然药物开发的支持。
