Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
CO.DON AG, Teltow, Germany.
Methods Mol Biol. 2023;2598:21-27. doi: 10.1007/978-1-0716-2839-3_3.
In native healthy hyaline cartilage, the chondrocytes are surrounded by a pericellular matrix that has a distinct composition and function compared to the hyaline cartilage extracellular matrix. The chondrocyte together with its pericellular matrix is called a chondron. The type VI collagen, which is the main component of the pericellular matrix, is resistant to enzymatic digestion by pure collagenase and dispase that do digest the extracellular matrix. Therefore, this combination of enzymes can be used to enzymatically isolate chondrons from hyaline cartilage. Chondrons have a high potential for cartilage tissue engineering. This chapter describes in detail how chondrons can be isolated from hyaline cartilage for further use.
在健康的天然透明软骨中，软骨细胞被细胞外基质包围，其组成和功能与透明软骨细胞外基质明显不同。软骨细胞与其细胞外基质一起被称为软骨小体。细胞外基质的主要成分 VI 型胶原对纯胶原酶和Dispase 的酶消化具有抗性，而Dispase 可消化细胞外基质。因此，这种酶的组合可用于从透明软骨中酶分离软骨小体。软骨小体具有很高的软骨组织工程潜力。本章详细描述了如何从透明软骨中分离软骨小体以供进一步使用。
