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人骨髓间充质基质细胞的分离和体外软骨分化。

Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.

机构信息

AO Research Institute Davos, Davos Platz, Switzerland.

UCB Pharma, Slough, UK.

出版信息

Methods Mol Biol. 2023;2598:65-73. doi: 10.1007/978-1-0716-2839-3_6.

DOI:10.1007/978-1-0716-2839-3_6
PMID:36355285
Abstract

Bone marrow-derived mesenchymal stromal cells (BM-MSC) are widely studied in the field of cartilage regeneration due to their capacity to differentiate into chondrocytes under specific in vitro culture conditions. This chapter describes the isolation of MSC from bone marrow aspirate, their expansion in monolayer, and the chondrogenic differentiation in pellet culture.

摘要

骨髓间充质基质细胞（BM-MSC）因其在特定的体外培养条件下能够分化为软骨细胞而在软骨再生领域得到广泛研究。本章描述了从骨髓抽吸物中分离 MSC、单层扩增和球状体培养中的软骨分化。

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本文引用的文献

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The edge effect: A global problem. The trouble with culturing cells in 96-well plates.边缘效应：一个全球性问题。在96孔板中培养细胞的问题。
Biochem Biophys Rep. 2021 Mar 25;26:100987. doi: 10.1016/j.bbrep.2021.100987. eCollection 2021 Jul.
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Predicting and Promoting Human Bone Marrow MSC Chondrogenesis by Way of TGFβ Receptor Profiles: Toward Personalized Medicine.通过转化生长因子β受体谱预测和促进人骨髓间充质干细胞软骨生成：迈向个性化医疗
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β-TCP from 3D-printed composite scaffolds acts as an effective phosphate source during osteogenic differentiation of human mesenchymal stromal cells.3D打印复合支架中的β-磷酸三钙在人间充质基质细胞成骨分化过程中作为有效的磷源。
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