Musculoskeletal Gene Therapy Laboratory, Mayo Clinic, Rochester, MN, USA.
Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN, USA.
Methods Mol Biol. 2023;2598:289-300. doi: 10.1007/978-1-0716-2839-3_20.
Viral gene transfer, known as transduction, is a powerful research tool for studying the biology of chondrocytes in novel ways and also a technology enabling the use of gene therapy for regenerating cartilage and treating diseases that affect cartilage, such as osteoarthritis. Adenovirus, retrovirus, lentivirus, and adeno-associated virus (AAV) are most commonly used to transduce chondrocytes. Although AAV is able to transduce chondrocytes in situ by intra-articular injection, chondrocytes are most commonly transduced in monolayer culture using the four vectors mentioned above. Protocols for achieving this are described, along with a discussion of the variables that can influence transduction efficiency.
病毒基因转移，又称转导，是一种强大的研究工具，可用于以新颖的方式研究软骨细胞的生物学特性，也是一种基因治疗技术，可用于再生软骨和治疗影响软骨的疾病，如骨关节炎。腺病毒、逆转录病毒、慢病毒和腺相关病毒（AAV）最常用于转导软骨细胞。虽然 AAV 可以通过关节内注射在原位转导软骨细胞，但最常用于使用上述四种载体在单层培养中转导软骨细胞。本文描述了实现这一目标的方案，并讨论了可能影响转导效率的变量。
