兔替代补体途径备解素的分离与鉴定

Isolation and characterization of rabbit properdin of the alternative complement pathway.

作者信息

Nakano Y, Matsuda T, Sakamoto T, Tomita M

出版信息

J Immunol Methods. 1986 Jun 10;90(1):77-83. doi: 10.1016/0022-1759(86)90386-8.

Abstract

Rabbit factor P, which is involved in stabilizing the labile C3 convertase of the alternative complement pathway, was isolated from rabbit serum by a simple two-step procedure: polyethylene glycol precipitation and QAE-Sephadex A-50 column chromatography. The chemical properties, and functional activities of the rabbit P were very similar to those of human P, except that there were slight differences in the SDS-PAGE patterns of limited tryptic digests and in the amino terminal sequences. The molecular weights were estimated to be 58 000 for rabbit P and 59 000 for human P on SDS-PAGE under reducing conditions. The amino terminal 36 residues of rabbit P showed 78% homology to the equivalent region of human P.

摘要

参与稳定替代补体途径不稳定C3转化酶的兔P因子，通过简单的两步法从兔血清中分离出来：聚乙二醇沉淀和QAE-葡聚糖凝胶A-50柱色谱法。兔P的化学性质和功能活性与人P非常相似，只是在有限胰蛋白酶消化的SDS-PAGE图谱和氨基末端序列上存在细微差异。在还原条件下进行SDS-PAGE时，兔P的分子量估计为58000，人P的分子量估计为59000。兔P的氨基末端36个残基与人P的等效区域显示出78%的同源性。