极化 P(VDF-TrFE) 薄膜通过可控表面电势促进皮肤伤口愈合。

Polarized P(VDF-TrFE) film promotes skin wound healing through controllable surface potential.

机构信息

Department of Rehabilitation Medicine, First Affiliated Hospital, College of Medicine, School of Materials Science and Engineering, Center of Rehabilitation Biomedical Materials, Zhejiang University, Hangzhou, Zhejiang Province, 310003, China; The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

出版信息

Colloids Surf B Biointerfaces. 2023 Jan;221:112980. doi: 10.1016/j.colsurfb.2022.112980. Epub 2022 Oct 28.

DOI:10.1016/j.colsurfb.2022.112980

PMID:36356402

Abstract

Surface potential of biomaterials is found to be important for wound healing. Here, poly(vinylidenefluoride-co-trifluoroethylene) (P(VDF-TrFE)) films with different surface potentials and piezoelectric responses were prepared and explored for the effect of surface potential on wound healing. The crystalline state of P(VDF-TrFE) films were characterized with X-ray diffraction (XRD), differential scanning calorimetry (DSC) and Fourier-transformed infrared spectroscopy (FTIR), illustrated that the electric polarization will promote the crystallization of the β phase of P(VDF-TrFE), in which the content of β phase increased from 82.9 % to 86.8 % compared with the control. Then, Kelvin potential and piezoelectric coefficient d were to evaluate surface potential and polarization performance. Moreover, bovine serum albumin (BSA) adsorption and cell culture results showed that high surface potential can promote protein adsorption as well as fibroblast proliferation and macrophage polarization. Finally, in vivo experiments indicated that high voltage polarized P(VDF-TrFE) films can generate higher dynamic potential up to 2.3 V, and promoted wound healing from the phases of inflammation, proliferation and remodeling, the wound healing rate of which was 88.8 % ± 0.8 %, significantly higher than 79.1 % ± 2.5 % and 86.4 % ± 1.8 % of blank and control. In general, this work revealed that polarized P(VDF-TrFE) films can promote wound healing, shed light on designing wound healing materials with similar properties.

摘要

生物材料的表面电势对于伤口愈合很重要。在这里，我们制备了具有不同表面电势和压电响应的聚偏二氟乙烯-三氟乙烯（P(VDF-TrFE))薄膜，并探索了表面电势对伤口愈合的影响。通过 X 射线衍射（XRD）、差示扫描量热法（DSC）和傅里叶变换红外光谱（FTIR）对 P(VDF-TrFE)薄膜的结晶状态进行了表征，结果表明电极化会促进 P(VDF-TrFE)的β相结晶，其中β相的含量从 82.9%增加到 86.8%。然后，用开尔文电势和压电系数 d 来评估表面电势和极化性能。此外，牛血清白蛋白（BSA）吸附和细胞培养结果表明，高表面电势可以促进蛋白质吸附以及成纤维细胞增殖和巨噬细胞极化。最后，体内实验表明，高压极化的 P(VDF-TrFE)薄膜可以产生高达 2.3V 的动态电势，并促进炎症、增殖和重塑阶段的伤口愈合，其愈合率为 88.8%±0.8%，明显高于空白组的 79.1%±2.5%和对照组的 86.4%±1.8%。总的来说，这项工作揭示了极化的 P(VDF-TrFE)薄膜可以促进伤口愈合，为设计具有类似性能的伤口愈合材料提供了思路。