Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Department of Stomatology, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361101, China.
ACS Appl Mater Interfaces. 2024 Jun 5;16(22):28029-28040. doi: 10.1021/acsami.4c00769. Epub 2024 May 22.
Biophysical and biochemical cues of biomaterials can regulate cell behaviors. Dental pulp stem cells (DPSCs) in pulp tissues can differentiate to odontoblast-like cells and secrete reparative dentin to form a barrier to protect the underlying pulp tissues and enable complete pulp healing. Promotion of the odontogenic differentiation of DPSCs is essential for dentin regeneration. The effects of the surface potentials of biomaterials on the adhesion and odontogenic differentiation of DPSCs remain unclear. Here, poly(vinylidene fluoride-trifluoro ethylene) (P(VDF-TrFE)) films with different surface potentials were prepared by the spin-coating technique and the contact poling method. The cytoskeletal organization of DPSCs grown on P(VDF-TrFE) films was studied by immunofluorescence staining. Using atomic force microscopy (AFM), the lateral detachment forces of DPSCs from P(VDF-TrFE) films were quantified. The effects of electrical stimulation generated from P(VDF-TrFE) films on odontogenic differentiation of DPSCs were evaluated and . The unpolarized, positively polarized, and negatively polarized films had surface potentials of -52.9, +902.4, and -502.2 mV, respectively. DPSCs on both negatively and positively polarized P(VDF-TrFE) films had larger cell areas and length-to-width ratios than those on the unpolarized films ( < 0.05). During the detachment of DPSCs from P(VDF-TrFE) films, the average magnitudes of the maximum detachment forces were 29.4, 72.1, and 53.9 nN for unpolarized, positively polarized, and negatively polarized groups, respectively ( < 0.05). The polarized films enhanced the mineralization activities and increased the expression levels of the odontogenic-related proteins of DPSCs compared to the unpolarized films ( < 0.05). The extracellular signal-regulated kinase (ERK) signaling pathway was involved in the odontogenic differentiation of DPSCs as induced by surface charge. , the polarized P(VDF-TrFE) films enhanced adhesion of DPSCs and promoted the odontogenic differentiation of DPSCs by electrical stimulation, demonstrating a potential application of electroactive biomaterials for reparative dentin formation in direct pulp capping.
生物材料的物理化学线索可以调节细胞行为。牙髓组织中的牙髓干细胞（DPSCs）可以分化为成牙本质细胞样细胞，并分泌修复性牙本质，形成屏障以保护下方的牙髓组织，并实现完全的牙髓愈合。促进 DPSCs 的牙向分化对于牙本质再生至关重要。生物材料表面电位对 DPSCs 黏附和牙向分化的影响尚不清楚。本研究采用旋涂技术和接触极化法制备了具有不同表面电位的聚（偏二氟乙烯-三氟乙烯）（P（VDF-TrFE））薄膜。通过免疫荧光染色研究了 DPSCs 在 P（VDF-TrFE）薄膜上的细胞骨架组织。通过原子力显微镜（AFM）定量测量 DPSCs 从 P（VDF-TrFE）薄膜上的侧向分离力。评估了 P（VDF-TrFE）薄膜产生的电刺激对 DPSCs 牙向分化的影响。未极化、正极化和负极化薄膜的表面电位分别为-52.9、+902.4 和-502.2 mV。与未极化薄膜相比，DPSCs 在正、负极化 P（VDF-TrFE）薄膜上的细胞面积和长宽比更大（<0.05）。在 DPSCs 从 P（VDF-TrFE）薄膜上脱落后，未极化、正极化和负极化组的最大分离力的平均大小分别为 29.4、72.1 和 53.9 nN（<0.05）。与未极化薄膜相比，极化薄膜增强了 DPSCs 的矿化活性，并增加了 DPSCs 牙向相关蛋白的表达水平（<0.05）。细胞外信号调节激酶（ERK）信号通路参与了表面电荷诱导的 DPSCs 牙向分化。综上所述，极化 P（VDF-TrFE）薄膜通过电刺激增强了 DPSCs 的黏附，并促进了 DPSCs 的牙向分化，为直接盖髓中修复性牙本质的形成提供了一种有潜力的电活性生物材料应用。
