Grupo Biología de Sistemas, Escuela de Ciencias de la Salud, Facultad de Medicina, Universidad Pontificia Bolivariana, Medellín, Antioquia, Colombia.
Grupo de Investigación en Salud Pública, Escuela de Ciencias de la Salud, Facultad de Medicina, Universidad Pontificia Bolivariana, Medellín, Antioquia, Colombia.
Comp Immunol Microbiol Infect Dis. 2022 Nov-Dec;90-91:101900. doi: 10.1016/j.cimid.2022.101900. Epub 2022 Nov 2.
This study aimed to determine the frequency of Babesia spp. infection in cattle, livestock farmers, and patients with acute febrile illness (AFI) from the Magdalena Medio region in Colombia using molecular and serological methods. PCR detected Babesia in 83.9 % (161/192) of cattle and 14.8 % (21/143) of farmers tested. Molecular analysis based on eight DNA sequences from the 18S rRNA identified Babesia bovis and Babesia bigemina in cattle and Babesia bigemina in farmers. There was no molecular detection in the patients with acute febrile illness; nonetheless, the serological test in the AFI population yielded 10.7 % (23/215) seropositivity for Babesia microti. Our findings suggest natural infection by this hemoparasite in this livestock region, and it is, therefore, essential to continue determining the role of this parasite as an etiological agent of diseases in the area, not only because of its veterinary relevance but also because of its zoonotic potential.
本研究旨在使用分子和血清学方法，确定哥伦比亚 Magdalena Medio 地区的牛、家畜饲养者和急性发热性疾病（AFI）患者中巴贝虫属感染的频率。PCR 检测到 192 头牛中有 83.9%（161/192）和 143 个农场主中有 14.8%（21/143）感染了巴贝虫。基于 18S rRNA 的 8 个 DNA 序列的分子分析在牛中鉴定出了巴贝斯虫和双芽巴贝斯虫，在农场主中鉴定出了双芽巴贝斯虫。急性发热性疾病患者中未进行分子检测；然而，在 AFI 人群中的血清学检测显示，巴贝斯虫微孢子虫的血清阳性率为 10.7%（23/215）。我们的研究结果表明，这种血液寄生虫在该畜牧区存在自然感染，因此，必须继续确定该寄生虫作为该地区疾病病因的作用，这不仅因为其具有兽医相关性，而且因为其具有潜在的人畜共患性。
