Platt David C, Apuzzo C Fiore, Jones Marjorie A, Cedeno David L, Vallejo Ricardo
Department of Chemistry, Illinois State University, Normal, IL 61790, USA.
Division of Research, SGX Medical LLC, Bloomington, IL 61701, USA.
Brain Sci. 2022 Nov 6;12(11):1504. doi: 10.3390/brainsci12111504.
This research focused on the development of an astrocyte cell model system (C6 glioma) for the assessment of molecular changes in response to cathodic passively balanced pulsed electrical stimulation at a rate of 50 Hz (60 µs duration, 0.15 mA intensity). Cells treated with selected neurotransmitters (glutamate, adenosine, D-serine, and γ-aminobutyric acid) were monitored (using specific fluorescent probes) for changes in levels of intracellular nitric oxide, calcium ions, and/or chloride. ES exerted an inhibitory effect on NO, increased calcium and had no effect on chloride. Using this model, cells can be assessed qualitatively and quantitatively for changes and these changes can be correlated with the putative molecular effects that electrical stimulation has on astrocytes and their role in glia-mediated diseases. This model system allows for faster and cheaper experiments than those involving animal models due to the potential to easily vary the conditions, reduce the number of variables (especially problematic in animal models), and closely monitor the cellular effects.
本研究聚焦于开发一种星形胶质细胞模型系统（C6胶质瘤），用于评估在50Hz（持续时间60微秒，强度0.15毫安）的阴极被动平衡脉冲电刺激下的分子变化。用选定的神经递质（谷氨酸、腺苷、D-丝氨酸和γ-氨基丁酸）处理的细胞（使用特定荧光探针）被监测细胞内一氧化氮、钙离子和/或氯离子水平的变化。电刺激对一氧化氮有抑制作用，增加钙离子水平，对氯离子无影响。使用该模型，可以对细胞变化进行定性和定量评估，并且这些变化可以与电刺激对星形胶质细胞的假定分子效应及其在胶质细胞介导疾病中的作用相关联。由于能够轻松改变条件、减少变量数量（这在动物模型中尤其成问题）并密切监测细胞效应，该模型系统比涉及动物模型的实验能够进行更快、更便宜的实验。
