Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Biomolecules. 2022 Oct 29;12(11):1591. doi: 10.3390/biom12111591.
Small-angle neutron scattering (SANS) is a powerful tool for studying biological membranes and model lipid bilayer membranes. The length scales probed by SANS, being from 1 nm to over 100 nm, are well-matched to the relevant length scales of the bilayer, particularly when it is in the form of a vesicle. However, it is the ability of SANS to differentiate between isotopes of hydrogen as well as the availability of deuterium labeled lipids that truly enable SANS to reveal details of membranes that are not accessible with the use of other techniques, such as small-angle X-ray scattering. In this work, an overview of the use of SANS for studying unilamellar lipid bilayer vesicles is presented. The technique is briefly presented, and the power of selective deuteration and contrast variation methods is discussed. Approaches to modeling SANS data from unilamellar lipid bilayer vesicles are presented. Finally, recent examples are discussed. While the emphasis is on studies of unilamellar vesicles, examples of the use of SANS to study intact cells are also presented.
小角中子散射（SANS）是研究生物膜和模型脂质双层膜的有力工具。SANS 探测的长度尺度为 1nm 到 100nm 以上，与双层膜的相关长度尺度非常匹配，特别是当它呈囊泡形式时。然而，SANS 能够区分氢的同位素，以及可用的氘标记脂质，这真正使 SANS 能够揭示其他技术（如小角 X 射线散射）无法获得的关于膜的细节。在这项工作中，介绍了 SANS 用于研究单层脂质双层囊泡的应用概述。简要介绍了该技术，并讨论了选择性氘化和对比变化方法的优势。介绍了从单层脂质双层囊泡建模 SANS 数据的方法。最后，讨论了最近的一些例子。虽然重点是研究单层囊泡，但也介绍了使用 SANS 研究完整细胞的例子。
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