使用 H NMR 和 TR-SANS 研究不对称脂质体中的脂双层翻转。

Studying lipid flip-flop in asymmetric liposomes using H NMR and TR-SANS.

机构信息

Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.

Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada; Department of Physics, University of Windsor, Windsor, ON, Canada.

出版信息

Methods Enzymol. 2024;700:295-328. doi: 10.1016/bs.mie.2024.02.012. Epub 2024 Mar 14.

DOI:10.1016/bs.mie.2024.02.012

PMID:38971604

Abstract

The specific spatial and temporal distribution of lipids in membranes play a crucial role in determining the biochemical and biophysical properties of the system. In nature, the asymmetric distribution of lipids is a dynamic process with ATP-dependent lipid transporters maintaining asymmetry, and passive transbilayer diffusion, that is, flip-flop, counteracting it. In this chapter, two probe-free techniques, H NMR and time-resolved small angle neutron scattering, are described in detail as methods of investigating lipid flip-flop rates in synthetic liposomes that have been generated with an asymmetric bilayer composition.

摘要

脂质在膜中的特定时空分布在决定系统的生化和生物物理性质方面起着至关重要的作用。在自然界中，脂质的不对称分布是一个动态过程，ATP 依赖性脂质转运蛋白维持其不对称性，而被动跨膜扩散，即翻转，与之对抗。在本章中，详细描述了两种无探针技术，即 H NMR 和时间分辨小角中子散射，作为研究具有不对称双层组成的合成脂质体中脂质翻转速率的方法。