Schwarz Markus L, Reisig Gregor, Schneider-Wald Barbara, Weiß Christel, Hauk Luisa, Schütte Andy
Section for Experimental Orthopaedics and Trauma Surgery, Orthopaedic and Trauma Surgery Centre (OUZ), Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Department of Medical Statistics, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Biomedicines. 2022 Oct 24;10(11):2685. doi: 10.3390/biomedicines10112685.
The coefficient of friction (CoF) serves as an indicator for the mechanical properties of natural and regenerated articular cartilage (AC). After tribological exposure, a height loss (HL) of the cartilage pair specimens can be measured. Our aim was to determine the CoF and HL of regenerated AC tissue and compare them with those of natural AC from non-operated joints and AC from joints where the regenerated tissues had been created after different treatments.
In partial-thickness defects of the trochleae of the stifle joints of 60 Göttingen Minipigs, regenerated AC was created. In total, 40 animals received a Col I matrix, 20 laden with autologous chondrocytes, and 20 without. The defects of 20 animals were left empty. The healing periods were 24 and 48 weeks. A total of 10 not-operated animals, delivered the "external" control specimens. Osteochondral pins were harvested from defect and non-defect areas, the latter serving as "internal" controls. Using a pin-on-plate tribometer, we measured the CoF and the HL.
The CoF of the regenerated AC ranged from 0.0393 to 0.0688, and the HL, from 0.22 mm to 0.3 mm. The differences between the regenerated AC of the six groups and the "external" controls were significant. The comparison with the "internal" controls revealed four significant differences for the CoF and one for the HL in the operated groups. No differences were seen within the operated groups.
The mechanical quality of the regenerated AC tissue showed inferior behavior with regard to the CoF and HL in comparison with natural AC. The comparison of regenerated AC tissue with AC from untreated joints was more promising than with AC from the treated joints.
摩擦系数（CoF）是天然和再生关节软骨（AC）力学性能的一个指标。经过摩擦学暴露后，可以测量软骨对标本的高度损失（HL）。我们的目的是确定再生AC组织的CoF和HL，并将其与未手术关节的天然AC以及经过不同处理后产生再生组织的关节的AC进行比较。
在60只哥廷根小型猪的膝关节滑车的部分厚度缺损处创建再生AC。总共40只动物接受了I型胶原基质，其中20只加载了自体软骨细胞，20只未加载。20只动物的缺损处保持为空。愈合期为24周和48周。总共10只未手术的动物提供了“外部”对照标本。从缺损和非缺损区域采集骨软骨销，后者作为“内部”对照。使用销盘摩擦计，我们测量了CoF和HL。
再生AC的CoF范围为0.0393至0.0688，HL范围为0.22毫米至0.3毫米。六组再生AC与“外部”对照之间的差异具有统计学意义。与“内部”对照相比，手术组在CoF方面有四个显著差异，在HL方面有一个显著差异。手术组内未见差异。
与天然AC相比，再生AC组织的力学质量在CoF和HL方面表现较差。将再生AC组织与未处理关节的AC进行比较比与处理过的关节的AC进行比较更有前景。
