Cuenca-Ortolá Irene, Martínez-Rojas Beatriz, Moreno-Manzano Victoria, García Castelló Marcos, Monleón Pradas Manuel, Martínez-Ramos Cristina, Más Estellés Jorge
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Cno. de Vera s/n, 46022 Valencia, Spain.
Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012 Valencia, Spain.
Biomedicines. 2022 Oct 28;10(11):2736. doi: 10.3390/biomedicines10112736.
Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury.
神经祖细胞（NPCs）已被证明在包括脊髓损伤治疗在内的不同细胞治疗方法中是一种有效的治疗策略。尽管有报道称NPC移植具有有益效果，但其低存活率和分化率构成了重要限制。在此，已开发出一种新方法，通过对接种在排列的聚乳酸纳米纤维支架上的NPC施加无线电刺激和磁刺激的组合，在移植前进行体外细胞预处理，以克服这两个限制。测试并比较了两种刺激模式，连续（每天施加一次长刺激）和间歇（每天施加三次短刺激）。结果表明，施加连续刺激可促进NPC增殖，并优先分化为少突胶质细胞和神经元谱系。与未刺激的NPC相比，观察到了神经样表型诱导。相比之下，间歇刺激模式不影响NPC增殖和向少突胶质细胞或星形胶质细胞形态的分化，但对神经元分化有不利影响。本研究提供了一种使用电刺激和磁刺激组合来诱导增殖和进一步神经元分化的新方法，这将改善脊髓损伤等疾病的治疗效果。
