Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, Hungary.
Department of Urology, Faculty of Medicine, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, Hungary.
Int J Mol Sci. 2022 Oct 27;23(21):13060. doi: 10.3390/ijms232113060.
Given the rising prevalence of lipid metabolic disorders and malignant diseases, we aimed to establish an in vivo hypercholesterinaemic tumour-bearing rat model for the induction and assessment of these conditions. A normal standard CRLT/N, 2 (baseline),- or 4 (2 + 2, pretreated)-week-long butter and cholesterol rich (BCR) diet was applied to mesoblastic nephroma (Ne/De) and myelomonoblastic leukaemia (My1/De) tumour-bearing and healthy control Long—Evans and Fischer 344 rats. The beginning of chow administration started in parallel with tumour induction and the 2 weeks of pre-transplantation in the baseline and pretreated groups, respectively. Fourteen days post-inoculation, the measurement of lipid parameters and [18F]F-FDG PET/MRI examinations was executed. The comparable lipid status of baseline healthy and tumorous rats proves that regardless of tumour presence, BCR-based hypercholesterolemia was achieved. A higher tumour mass among pretreated tumorous animals was found when compared to the control groups (p < 0.05, p < 0.01). Further, a visually greater [18F]F-FDG accumulation was observed in pretreated BCR tumorous animals; however, the quantitative data (SUVmean: 9.86 ± 0.98, 9.68 ± 1.24; SUVmax: 19.63 ± 1.20; 17.56 ± 3.21 for Ne/De and My1/De, respectively) were not statistically significantly different from those of the CRLT/N tumorous rats (SUVmean: 8.40 ± 1.42, 7.22 ± 1.06 and SUVmax: 15.99 ± 2.22, 12.46 ± 1.96 for control Ne/De and My1/De, respectively). Our model seems to be appropriate for simultaneously investigating hypercholesterolemia and cancer in the same rat.
鉴于脂质代谢紊乱和恶性肿瘤的发病率不断上升，我们旨在建立一种体内高胆固醇血症荷瘤大鼠模型，以诱导和评估这些疾病。将正常标准的 CRLT/N、2（基线）或 4（2+2，预处理）周长黄油和胆固醇丰富（BCR）饮食应用于间充质肾瘤（Ne/De）和髓单核白血病（My1/De）荷瘤和健康对照的长耳白和 Fischer 344 大鼠。在基线和预处理组中，分别在肿瘤诱导的同时和移植前的 2 周开始给予饲料。接种后 14 天，进行脂质参数测量和 [18F]F-FDG PET/MRI 检查。基线健康和肿瘤大鼠相似的脂质状态证明，无论肿瘤是否存在，都可以通过 BCR 实现高胆固醇血症。与对照组相比，预处理肿瘤动物的肿瘤质量更高（p < 0.05，p < 0.01）。此外，预处理 BCR 肿瘤动物的 [18F]F-FDG 积累明显更多；然而，定量数据（SUVmean：9.86 ± 0.98，9.68 ± 1.24；SUVmax：19.63 ± 1.20；17.56 ± 3.21 分别为 Ne/De 和 My1/De，而 CRLT/N 肿瘤大鼠的 SUVmean：8.40 ± 1.42，7.22 ± 1.06 和 SUVmax：15.99 ± 2.22，12.46 ± 1.96 分别为对照 Ne/De 和 My1/De）没有统计学意义。我们的模型似乎适合同时在同一只大鼠中研究高胆固醇血症和癌症。
