Yovinska Svetlana, Belemezova Kalina, Hristova-Savova Mariela, Milachich Tanya, Andreeva Petya, Veleva Lachezara, Buchvarov Yuri, Yunakova Maria, Timeva Tanya, Shterev Atanas, Dimova Ivanka
Department of Pharmacology and Toxicology, Medical University Sofia, 1431 Sofia, Bulgaria.
SAGBAL 'Dr Shterev', 1330 Sofia, Bulgaria.
Life (Basel). 2022 Nov 9;12(11):1840. doi: 10.3390/life12111840.
The aim of our study was to determine the type and frequency of chromosomal aberrations and polymorphisms in men with different degrees of spermatogenic failure in comparison to men with normozoospermia, in order to find correlations between cytogenetic findings and the abnormal results of semen analysis. In our study, we performed cytogenetic analysis in 901 infertile men, divided into five groups according to semen analysis-normozoospermia (86), asthenozoospermia (394), oligoasthenozoospermia (182), severe male factor (100), and azoospermia (139). The frequency of polymorphisms was similar in all groups (11-16%, without significant differences). The frequency of numerical and structural aberrations increases with the degree of the spermatogenic failure (3.5% in normozoospermia, 5.6% in asthenozoospermia, 9.8% in oligoasthenozoospermia, 9% in severe male factor, and 13.5% in azoospermia). We found a significantly higher incidence of numerical chromosomal aberrations in severe male factor (7%) and azoospermia (9.3%). Oligoasthenozoospermia occured in 45% of cases with translocation, compared to 20% in the group with a normal karyotype. We revealed that chromosomal translocations are tightly associated with oligoasthenozoospermia, whereas numerical chromosomal aberrations-with severe male factor and azoospermia. The impact of chromosome polymorphisms on male infertility should be studied in greater detail.
我们研究的目的是确定不同程度生精功能障碍男性与正常精子症男性相比，染色体畸变和多态性的类型及频率，以找出细胞遗传学结果与精液分析异常结果之间的相关性。在我们的研究中，我们对901名不育男性进行了细胞遗传学分析，根据精液分析将其分为五组：正常精子症（86例）、弱精子症（394例）、少弱精子症（182例）、严重男性因素（100例）和无精子症（139例）。所有组中多态性的频率相似（11 - 16%，无显著差异）。数值和结构畸变的频率随生精功能障碍程度的增加而升高（正常精子症中为3.5%，弱精子症中为5.6%，少弱精子症中为9.8%，严重男性因素中为9%，无精子症中为13.5%）。我们发现严重男性因素组（7%）和无精子症组（9.3%）中数值染色体畸变的发生率显著更高。少弱精子症在45%的易位病例中出现，而正常核型组为20%。我们发现染色体易位与少弱精子症密切相关，而数值染色体畸变与严重男性因素和无精子症密切相关。染色体多态性对男性不育的影响应进行更详细的研究。
