Suzaki T, Williamson R E
J Cell Sci. 1986 Feb;80:75-89. doi: 10.1242/jcs.80.1.75.
Detergent-extracted cell models of the euglenoid flagellate, Astasia longa, were obtained that rounded-up on addition of calcium. Treatment with 4% Triton X-100 and Nonidet P-40 removed the flagellar membrane, all membranous structures inside the cell body and the plasma membrane at groove regions of the cell surface. Maximum rounding-up was induced when the concentration of free calcium was raised to greater than or equal to 10(-7) M, and ATP strongly enhanced this response. The ionic requirements and sensitivity to vanadate were different from those for the reactivation of flagellar movement. The results suggest that the mechanism of force generation is different from the dynein-based system of the flagellum and that a rise in cytoplasmic free Ca2+ concentration might cause euglenoid movement in vivo. The mechanism of euglenoid movement is discussed in relation to other protozoan motile systems.
获得了裸藻鞭毛虫长眼虫（Astasia longa）的去污剂提取细胞模型，该模型在添加钙后会变圆。用4% Triton X - 100和Nonidet P - 40处理可去除鞭毛膜、细胞体内所有膜结构以及细胞表面沟区的质膜。当游离钙浓度升高到大于或等于10^(-7) M时，诱导出最大程度的变圆，并且ATP强烈增强了这种反应。离子需求和对钒酸盐的敏感性与鞭毛运动重新激活的情况不同。结果表明，力产生机制不同于基于动力蛋白的鞭毛系统，并且细胞质游离Ca2+浓度的升高可能在体内引起裸藻运动。本文结合其他原生动物运动系统讨论了裸藻运动的机制。
