水溶性低分子量乙酰氨基葡萄糖的制备及其对 H22 荷瘤小鼠的抗肿瘤活性。
Preparation of Water-Soluble Acetylaminoglucan with Low Molecular Weight and Its Anti-Tumor Activity on H22 Tumor-Bearing Mice.
机构信息
Key Laboratory of Industrial Fermentation Microbiology Ministry of Education & Tianjin Key Laboratory of Industrial Microbiology, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
State Key Laboratory of Food Nutrition and Safety Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
出版信息
Molecules. 2022 Oct 26;27(21):7273. doi: 10.3390/molecules27217273.
In this study, a novel low molecular weight of acetylaminoglucan (AGA) was obtained and its antitumor activity on H22 tumor-bearing mice was investigated. The results of UV, HPLC and FT-IR showed that AGA present high purity with low molecular weight of 2.76 × 10 Da. Animal experiments showed that AGA could inhibit the proliferation of tumor cells in H22 tumor-bearing mice by protecting the immune organs, enhancing the phagocytosis ability of macrophages, killing activity of NK cells and proliferation capacity of lymphocytes, improving the levels of cytokines in vivo and regulating the distribution of lymphocyte subsets, and the tumor inhibition rate reached to 52.74% (50 mg/kg). Cell cycle determination further indicated that AGA could induce apoptosis of tumor cells and arrests it in S phase. These results will provide a data basis for the potential application of AGA in pharmaceutical industry.
在这项研究中，获得了一种新型的低分子量乙酰氨基葡萄糖（AGA），并研究了其对 H22 荷瘤小鼠的抗肿瘤活性。紫外、高效液相色谱和傅里叶变换红外光谱的结果表明，AGA 具有高纯度和低分子量（2.76×10 Da）。动物实验表明，AGA 通过保护免疫器官、增强巨噬细胞的吞噬能力、NK 细胞的杀伤活性和淋巴细胞的增殖能力，提高体内细胞因子水平并调节淋巴细胞亚群的分布，可抑制 H22 荷瘤小鼠肿瘤细胞的增殖，肿瘤抑制率达到 52.74%（50mg/kg）。细胞周期测定进一步表明，AGA 可诱导肿瘤细胞凋亡并将其阻滞在 S 期。这些结果将为 AGA 在制药工业中的潜在应用提供数据基础。
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