Yakovlev Dmitry, Kolesova Ekaterina, Sizova Svetlana, Annas Kirill, Tretyak Marina, Loschenov Victor, Orlova Anna, Oleinikov Vladimir
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, 117997 Moscow, Russia.
Prokhorov General Physics Institute, Russian Academy of Science, 119991 Moscow, Russia.
Nanomaterials (Basel). 2022 Nov 2;12(21):3874. doi: 10.3390/nano12213874.
Today, fluorescent diagnostics and photodynamic therapy are promising methods for diagnosing and treating oncological diseases. The development of new photosensitizers (PS) is one of the most important tasks to improve the efficiency of both laser-induced diagnostics and therapy. In our study, we conjugated PS with AIS/ZnS triple quantum dots (QDs) to obtain non-aggregated complexes. It was shown that the conjugation of PS with QDs does not change the PS fluorescence lifetime, which is a marker of the preservation of PS photophysical properties. In particular, efficient resonant Förster energy transfer (FRET), from QDs to PS molecules in the conjugate, increases the PS luminescence response. The FRET from QD to PS molecules with different ratios of donor and acceptors are shown. It has been demonstrated that the average efficiency of FRET depends on the ratio of PS and QD and reaches a maximum value of 80% at a ratio of 6 PS molecules per 1 QD molecule. Thus, these studies could help to contribute to the development of new complexes based on QD and PS to improve the efficiency of phototheranostics.
如今，荧光诊断和光动力疗法是诊断和治疗肿瘤疾病的有前景的方法。开发新型光敏剂（PS）是提高激光诱导诊断和治疗效率的最重要任务之一。在我们的研究中，我们将PS与AIS/ZnS三量子点（QD）共轭以获得非聚集复合物。结果表明，PS与QD的共轭不会改变PS的荧光寿命，而荧光寿命是PS光物理性质保持的一个标志。特别是，从QD到共轭物中PS分子的高效共振Förster能量转移（FRET）增加了PS的发光响应。展示了不同供体和受体比例下从QD到PS分子的FRET。已经证明，FRET的平均效率取决于PS和QD的比例，并且在每1个QD分子对应6个PS分子的比例下达到最大值80%。因此，这些研究有助于推动基于QD和PS的新型复合物的开发，以提高光诊疗效率。
