Kurassova Kamilla, Filatov Nikita, Karamysheva Sofia, Bukatin Anton, Starovoytov Anton, Vartanyan Tigran, Vollmer Frank, Toropov Nikita A
International Research and Education Centre for Physics of Nanostructures, ITMO University, St. Petersburg 197101, Russia.
Alferov Saint Petersburg National Research Academic University of the Russian Academy of Sciences, 8/3A Khlopina Street, St. Petersburg 194021, Russia.
ACS Omega. 2024 Sep 2;9(37):39287-39295. doi: 10.1021/acsomega.4c07270. eCollection 2024 Sep 17.
Fluorescent microspheres are at the forefront of biosensing technologies. They can be used for a wide range of biomedical applications. They consist of organic dyes and polymers, which are relatively immune to photobleaching and other environmental factors. However, recently developed AgInS/ZnS quantum dots are a water-soluble, low-toxicity class of semiconductor nanocrystals with enhanced stability as fluorescent materials. Here, we propose a simple way for making microspheres: a microfluidic dripping technique for acrylamide polymer spheres doped with quantum dots. Analyses of their spectra show that the emission of quantum dots, dispersed in water, is saturated with an increasing pump intensity, while quantum dots embedded into polymer microspheres exhibit a more sustained emission. Moreover, our study unveils a remarkable reduction in the luminescence lifetime of quantum dots embedded in microspheres: the mean value of the decay time for quantum dots in solutions was 91 and 3.5 ns for similar quantum dots incorporated into polymer microspheres.
荧光微球处于生物传感技术的前沿。它们可用于广泛的生物医学应用。它们由有机染料和聚合物组成，相对不易受到光漂白和其他环境因素的影响。然而，最近开发的AgInS/ZnS量子点是一类水溶性、低毒性的半导体纳米晶体，作为荧光材料具有增强的稳定性。在这里，我们提出了一种制备微球的简单方法：一种用于掺杂量子点的丙烯酰胺聚合物微球的微流控滴注技术。对其光谱的分析表明，分散在水中的量子点的发射随着泵浦强度的增加而饱和，而嵌入聚合物微球中的量子点表现出更持续的发射。此外，我们的研究揭示了嵌入微球中的量子点的发光寿命显著降低：溶液中量子点的衰变时间平均值为91纳秒，而掺入聚合物微球中的类似量子点的衰变时间平均值为3.5纳秒。
