Cotuțiu Vlad-Dan, Mihalca Andrei Daniel, Hołówka Katarzyna Anna, Ionică Angela Monica, Cazan Cristina Daniela, Gherman Călin Mircea
Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.
Molecular Biology and Veterinary Parasitology Unit (CDS-9), University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.
Pathogens. 2022 Oct 24;11(11):1225. doi: 10.3390/pathogens11111225.
Thelaziosis caused by is an emerging disease in Europe. Only two reports of naturally infected lagomorphs have been published so far. The aim of this study was to evaluate the status of the Romanian populations of European brown hares, as reservoir hosts for . Between November 2019 and November 2021, the eyes of 326 carcasses were examined for the presence of ocular parasites. Nematodes were stored in plastic vials with physiological saline, followed by morphological and molecular identification. QGis 3.20 and EpiInfo 7 were used for mapping and statistical analysis. Four (1.23%) hares harbored infection, with a total of 84 nematodes collected (mean intensity 21 nematodes/host), with 45 males, 39 females (two sexually immature, seven with only eggs, and 30 with eggs and larvae). One specimen from each host was successfully sequenced resulting in a 100% similarity with several other sequences of haplotype 1. Statistical analysis revealed no significant results. The current study represents a first report of in the European brown hare in Romania, and the second in Europe, also reiterating the role of lagomorphs as reservoir hosts for this zoonotic ocular nematode.
由[病原体名称未给出]引起的吸吮线虫病在欧洲是一种新出现的疾病。迄今为止，仅发表了两篇关于自然感染兔形目动物的报告。本研究的目的是评估罗马尼亚欧洲棕兔种群作为[病原体名称未给出]储存宿主的状况。在2019年11月至2021年11月期间，检查了326具兔尸的眼睛，以确定是否存在眼部寄生虫。线虫保存在装有生理盐水的塑料小瓶中，随后进行形态学和分子鉴定。使用QGis 3.20和EpiInfo 7进行绘图和统计分析。四只（1.23%）野兔感染了[病原体名称未给出]，共收集到84条线虫（平均感染强度为21条线虫/宿主），其中45只为雄虫，39只为雌虫（两只性未成熟，七只仅含虫卵，30只含虫卵和幼虫）。成功对每只宿主的一个样本进行了测序，结果显示与单倍型1的其他几个序列具有100%的相似性。统计分析未得出显著结果。本研究是罗马尼亚欧洲棕兔感染[病原体名称未给出]的首次报告，也是欧洲的第二次报告，同时也再次强调了兔形目动物作为这种人畜共患眼部线虫储存宿主的作用。
