Thiebes Anja Lena, Klein Sarah, Zingsheim Jonas, Möller Georg H, Gürzing Stefanie, Reddemann Manuel A, Behbahani Mehdi, Jockenhoevel Stefan, Cornelissen Christian G
Department of Biohybrid & Medical Textiles (BioTex), AME-Institute of Applied Medical Engineering, Helmholtz Institute Aachen, RWTH Aachen University, Forckenbeckstraße 55, 52074 Aachen, Germany.
Aachen-Maastricht Institute for Biobased Materials, Faculty of Science and Engineering, Maastricht University, Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD Geleen, The Netherlands.
Pharmaceutics. 2022 Nov 9;14(11):2421. doi: 10.3390/pharmaceutics14112421.
Cell spraying has become a feasible application method for cell therapy and tissue engineering approaches. Different devices have been used with varying success. Often, twin-fluid atomizers are used, which require a high gas velocity for optimal aerosolization characteristics. To decrease the amount and velocity of required air, a custom-made atomizer was designed based on the effervescent principle. Different designs were evaluated regarding spray characteristics and their influence on human adipose-derived mesenchymal stromal cells. The arithmetic mean diameters of the droplets were 15.4−33.5 µm with decreasing diameters for increasing gas-to-liquid ratios. The survival rate was >90% of the control for the lowest gas-to-liquid ratio. For higher ratios, cell survival decreased to approximately 50%. Further experiments were performed with the design, which had shown the highest survival rates. After seven days, no significant differences in metabolic activity were observed. The apoptosis rates were not influenced by aerosolization, while high gas-to-liquid ratios caused increased necrosis levels. Tri-lineage differentiation potential into adipocytes, chondrocytes, and osteoblasts was not negatively influenced by aerosolization. Thus, the effervescent aerosolization principle was proven suitable for cell applications requiring reduced amounts of supplied air. This is the first time an effervescent atomizer was used for cell processing.
细胞喷雾已成为细胞治疗和组织工程方法的一种可行应用方式。人们使用了不同的设备，取得的成功程度各异。通常会使用双流雾化器，其需要较高的气体流速以获得最佳的雾化特性。为了减少所需空气的量和流速，基于泡腾原理设计了一种定制雾化器。针对喷雾特性及其对人脂肪来源间充质基质细胞的影响评估了不同设计。液滴的算术平均直径为15.4 - 33.5 µm，随着气液比增加直径减小。对于最低气液比，存活率高于对照组的90%。对于更高的气液比，细胞存活率降至约50%。对显示出最高存活率的设计进行了进一步实验。七天后，未观察到代谢活性有显著差异。凋亡率不受雾化影响，而高气液比导致坏死水平增加。向脂肪细胞、软骨细胞和成骨细胞的三系分化潜能不受雾化的负面影响。因此，泡腾雾化原理被证明适用于需要减少供气量的细胞应用。这是首次将泡腾雾化器用于细胞处理。
