School of Software, Hallym University, Chuncheon-si 24252, Korea.
Bio-IT Research Center, Hallym University, Chuncheon-si 24252, Korea.
Sensors (Basel). 2022 Nov 7;22(21):8575. doi: 10.3390/s22218575.
The polymerase chain reaction is an important technique in biological research. However, it is time consuming and has a number of disadvantages. Therefore, real-time PCR technology that can be used in real-time monitoring has emerged, and many studies are being conducted regarding its use. Real-time PCR requires many optical components and imaging devices such as expensive, high-performance cameras. Therefore, its cost and assembly process are limitations to its use. Currently, due to the development of smart camera devices, small, inexpensive cameras and various lenses are being developed. In this paper, we present a Compact Camera Fluorescence Detector for use in parallel-light lens-based real-time PCR devices. The proposed system has a simple optical structure, the system cost can be reduced, and the size can be miniaturized. This system only incorporates Fresnel lenses without additional optics in order for the same field of view to be achieved for 25 tubes. In the center of the Fresnel lens, one LED and a complementary metal-oxide semiconductor camera were placed in directions that were as similar as possible. In addition, to achieve the accurate analysis of the results, image processing was used to correct them. As a result of an experiment using a reference fluorescent substance and double-distilled water, it was confirmed that stable fluorescence detection was possible.
聚合酶链反应是生物研究中的一项重要技术。然而，它耗时且存在许多缺点。因此，能够实时监测的实时 PCR 技术应运而生，许多研究正在进行中。实时 PCR 需要许多光学组件和成像设备，如昂贵的高性能相机。因此，其成本和组装过程是其使用的限制因素。目前，由于智能相机设备的发展，正在开发小型、廉价的相机和各种镜头。在本文中，我们提出了一种用于基于平行光透镜的实时 PCR 设备的紧凑型相机荧光探测器。所提出的系统具有简单的光学结构，可以降低系统成本，并可以实现小型化。该系统仅包含菲涅耳透镜，而无需额外的光学元件，以便为 25 个管实现相同的视场。在菲涅耳透镜的中心，一个 LED 和一个互补金属氧化物半导体相机被放置在尽可能相似的方向。此外，为了实现结果的准确分析，使用图像处理对其进行了校正。通过使用参考荧光物质和双蒸水进行实验，证实了可以进行稳定的荧光检测。
