Kamil Mohd, Deveci Gozde, Kina Umit Y, Kappe Stefan H I, Aly Ahmed S I
Aly Lab., Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University, Istanbul 34820, Turkey.
Department of Biotechnology, Institute of Health Sciences, Bezmialem Vakif University, Istanbul 34093, Turkey.
Vaccines (Basel). 2022 Nov 8;10(11):1884. doi: 10.3390/vaccines10111884.
Host cell-free, axenic development of liver stages (LS) of the malaria parasite has been demonstrated. Here we explored axenic liver stages as a novel live whole parasite malaria vaccine platform, which is unaltered and not prone to human-error, compared to the immunization with live-attenuated sporozoites that must be done intravenously. We show that in contrast to live sporozoites, axenic LS are not infectious to the immunized host. Subcutaneous immunizations of mice with axenic LS, developed from wild-type (WT) sporozoites or WT sporozoites expressing enhanced-GFP, conferred sterile protection against infectious sporozoite challenge. Thus, axenic liver stages of and might constitute an attractive alternative to live sporozoite immunization.
疟原虫肝期（LS）在无宿主细胞、无菌条件下的发育已得到证实。在此，我们探索将无菌肝期作为一种新型的活全寄生虫疟疾疫苗平台，与必须通过静脉注射进行的减毒活子孢子免疫相比，该平台未发生改变且不易出现人为错误。我们发现，与活子孢子不同，无菌肝期对免疫宿主无感染性。用野生型（WT）子孢子或表达增强型绿色荧光蛋白的WT子孢子发育而来的无菌肝期对小鼠进行皮下免疫，可使其获得针对感染性子孢子攻击的无菌保护。因此，疟原虫和的无菌肝期可能是活子孢子免疫的一个有吸引力的替代方案。
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