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用于超分辨率显微镜的可控光子射流

Steerable photonic jet for super-resolution microscopy.

作者信息

Karamehmedović Mirza, Scheel Kenneth, Listov-Saabye Pedersen Frederik, Villegas Arturo, Hansen Poul-Erik

出版信息

Opt Express. 2022 Nov 7;30(23):41757-41773. doi: 10.1364/OE.472992.

Abstract

A promising technique in optical super-resolution microscopy is the illumination of the sample by a highly localized beam, a photonic jet (also called photonic nanojet). We propose a method of computation of incident field amplitude and phase profiles that produce photonic jets at desired locations in the near field after interaction with a fixed micro-scale dielectric lens. We also describe a practical way of obtaining the incident field profiles using spatial light modulators. We expect our photonic jet design method to work for a wide range of lens shapes, and we demonstrate its application numerically using two-dimensional micro-lenses of circular and square cross-sections. We furthermore offer a theoretical analysis of the resolution of photonic jet design, predicting among other that a larger lens can produce a narrower photonic jet. Finally, we give both theoretical and numerical evidence that the waist width of the achieved designed jets is increasing linearly and slowly over a large interval of radial distances. With uniform plane wave illumination, the circular two-dimensional micro-lens produces a similar-sized jet at a fixed radial distance, while the square lens does not form a jet at all. We expect our steerable optical photonic jet probe to enable highly localized adaptive real-time measurements and drive advances in super-resolution optical microscopy and scatterometry, as well as fluorescence and Raman microscopy. Our relatively weak peak jet intensity allows application in biology and health sciences, which require high resolution imaging without damaging the sample bio-molecules.

摘要

光学超分辨率显微镜中的一种很有前景的技术是用高度局域化的光束照射样品,即光子射流(也称为光子纳米射流)。我们提出了一种计算入射场振幅和相位分布的方法,该方法能在与固定的微尺度介电透镜相互作用后,在近场的期望位置产生光子射流。我们还描述了一种使用空间光调制器获得入射场分布的实用方法。我们期望我们的光子射流设计方法适用于多种透镜形状,并使用圆形和方形横截面的二维微透镜进行了数值演示。此外,我们对光子射流设计的分辨率进行了理论分析,预测了其他情况,即更大的透镜可以产生更窄的光子射流。最后,我们给出了理论和数值证据,表明在较大的径向距离区间内,所实现的设计射流的束腰宽度呈线性且缓慢增加。在均匀平面波照明下,圆形二维微透镜在固定径向距离处产生类似尺寸的射流,而方形透镜根本不形成射流。我们期望我们的可控光学光子射流探头能够实现高度局域化的自适应实时测量,并推动超分辨率光学显微镜、散射测量以及荧光和拉曼显微镜技术的发展。我们相对较弱的射流峰值强度允许其应用于生物学和健康科学领域,这些领域需要高分辨率成像而不损坏样品生物分子。

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