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用于超分辨率显微镜的可控光子射流

Steerable photonic jet for super-resolution microscopy.

作者信息

Karamehmedović Mirza, Scheel Kenneth, Listov-Saabye Pedersen Frederik, Villegas Arturo, Hansen Poul-Erik

出版信息

Opt Express. 2022 Nov 7;30(23):41757-41773. doi: 10.1364/OE.472992.

DOI:10.1364/OE.472992
PMID:36366644
Abstract

A promising technique in optical super-resolution microscopy is the illumination of the sample by a highly localized beam, a photonic jet (also called photonic nanojet). We propose a method of computation of incident field amplitude and phase profiles that produce photonic jets at desired locations in the near field after interaction with a fixed micro-scale dielectric lens. We also describe a practical way of obtaining the incident field profiles using spatial light modulators. We expect our photonic jet design method to work for a wide range of lens shapes, and we demonstrate its application numerically using two-dimensional micro-lenses of circular and square cross-sections. We furthermore offer a theoretical analysis of the resolution of photonic jet design, predicting among other that a larger lens can produce a narrower photonic jet. Finally, we give both theoretical and numerical evidence that the waist width of the achieved designed jets is increasing linearly and slowly over a large interval of radial distances. With uniform plane wave illumination, the circular two-dimensional micro-lens produces a similar-sized jet at a fixed radial distance, while the square lens does not form a jet at all. We expect our steerable optical photonic jet probe to enable highly localized adaptive real-time measurements and drive advances in super-resolution optical microscopy and scatterometry, as well as fluorescence and Raman microscopy. Our relatively weak peak jet intensity allows application in biology and health sciences, which require high resolution imaging without damaging the sample bio-molecules.

摘要

光学超分辨率显微镜中的一种很有前景的技术是用高度局域化的光束照射样品,即光子射流(也称为光子纳米射流)。我们提出了一种计算入射场振幅和相位分布的方法,该方法能在与固定的微尺度介电透镜相互作用后,在近场的期望位置产生光子射流。我们还描述了一种使用空间光调制器获得入射场分布的实用方法。我们期望我们的光子射流设计方法适用于多种透镜形状,并使用圆形和方形横截面的二维微透镜进行了数值演示。此外,我们对光子射流设计的分辨率进行了理论分析,预测了其他情况,即更大的透镜可以产生更窄的光子射流。最后,我们给出了理论和数值证据,表明在较大的径向距离区间内,所实现的设计射流的束腰宽度呈线性且缓慢增加。在均匀平面波照明下,圆形二维微透镜在固定径向距离处产生类似尺寸的射流,而方形透镜根本不形成射流。我们期望我们的可控光学光子射流探头能够实现高度局域化的自适应实时测量,并推动超分辨率光学显微镜、散射测量以及荧光和拉曼显微镜技术的发展。我们相对较弱的射流峰值强度允许其应用于生物学和健康科学领域,这些领域需要高分辨率成像而不损坏样品生物分子。

相似文献

1
Steerable photonic jet for super-resolution microscopy.用于超分辨率显微镜的可控光子射流
Opt Express. 2022 Nov 7;30(23):41757-41773. doi: 10.1364/OE.472992.
2
Photonic jets produced by dielectric micro cuboids.
Appl Opt. 2015 Oct 10;54(29):8694-9. doi: 10.1364/AO.54.008694.
3
Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet.介质微球的超分辨成像由其光子纳米射流的腰部决定。
Nano Lett. 2016 Aug 10;16(8):4862-70. doi: 10.1021/acs.nanolett.6b01255. Epub 2016 Jul 19.
4
Step-Index (Semi-Immersed) Model for Photonic Nanojet and Experimental Characterization via Near-Field Optical Microscopy with Microcylinder.光子纳米射流的阶跃折射率(半浸没)模型及通过带有微柱体的近场光学显微镜进行的实验表征
Nanomaterials (Basel). 2023 Mar 13;13(6):1033. doi: 10.3390/nano13061033.
5
Terahertz tunable three-dimensional photonic jets.太赫兹可调谐三维光子射流。
Sci Rep. 2024 Jul 17;14(1):16522. doi: 10.1038/s41598-024-64158-6.
6
Optical Force on a Metal Nanorod Exerted by a Photonic Jet.光子喷射对金属纳米棒施加的光学力。
Nanomaterials (Basel). 2022 Jan 13;12(2):251. doi: 10.3390/nano12020251.
7
Localized photonic jets from flat, three-dimensional dielectric cuboids in the reflection mode.反射模式下扁平三维介电长方体产生的局域光子射流
Opt Lett. 2015 May 15;40(10):2329-32. doi: 10.1364/OL.40.002329.
8
Terahertz scanning microscopy with 2λ depth of field based on photonic nanojet generated by a dielectric cuboid probe.基于介质方柱探头产生的光子纳米射流的 2λ 景深太赫兹扫描显微镜。
Opt Express. 2022 Dec 5;30(25):45303-45311. doi: 10.1364/OE.472209.
9
Photonic jet with ultralong working distance by hemispheric shell.由半球形外壳实现的具有超长工作距离的光子喷射。
Opt Express. 2015 Mar 9;23(5):6626-33. doi: 10.1364/OE.23.006626.
10
Phase-only steerable photonic nanojets.
Opt Express. 2023 Aug 14;31(17):27255-27265. doi: 10.1364/OE.497469.